DeamiDATE 1.0: Site-specific deamidation as a tool to assess authenticity of members of ancient proteomes

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

DeamiDATE 1.0 : Site-specific deamidation as a tool to assess authenticity of members of ancient proteomes. / Ramsøe, Abigail; van Heekeren, Vivian; Ponce, Paola; Fischer, Roman; Barnes, Ian; Speller, Camilla; Collins, Matthew J.

In: Journal of Archaeological Science, Vol. 115, 105080, 03.2020.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Ramsøe, A, van Heekeren, V, Ponce, P, Fischer, R, Barnes, I, Speller, C & Collins, MJ 2020, 'DeamiDATE 1.0: Site-specific deamidation as a tool to assess authenticity of members of ancient proteomes', Journal of Archaeological Science, vol. 115, 105080. https://doi.org/10.1016/j.jas.2020.105080

APA

Ramsøe, A., van Heekeren, V., Ponce, P., Fischer, R., Barnes, I., Speller, C., & Collins, M. J. (2020). DeamiDATE 1.0: Site-specific deamidation as a tool to assess authenticity of members of ancient proteomes. Journal of Archaeological Science, 115, [105080]. https://doi.org/10.1016/j.jas.2020.105080

Vancouver

Ramsøe A, van Heekeren V, Ponce P, Fischer R, Barnes I, Speller C et al. DeamiDATE 1.0: Site-specific deamidation as a tool to assess authenticity of members of ancient proteomes. Journal of Archaeological Science. 2020 Mar;115. 105080. https://doi.org/10.1016/j.jas.2020.105080

Author

Ramsøe, Abigail ; van Heekeren, Vivian ; Ponce, Paola ; Fischer, Roman ; Barnes, Ian ; Speller, Camilla ; Collins, Matthew J. / DeamiDATE 1.0 : Site-specific deamidation as a tool to assess authenticity of members of ancient proteomes. In: Journal of Archaeological Science. 2020 ; Vol. 115.

Bibtex

@article{5c5aca9c87dd4e1c82e88e47767868aa,
title = "DeamiDATE 1.0: Site-specific deamidation as a tool to assess authenticity of members of ancient proteomes",
abstract = "Contamination is a potential problem in the study of ancient proteins, either from prior handling of the sample, laboratory consumables, or cross-sample carryover from mass spectrometers. Recently, deamidation of glutamine has been proposed as a measure for assessing the degradation of ancient proteins. Here, we present deamiDATE 1.0, a method for the authentication of ancient proteins using measure of site-specific deamidation rates. We test this approach on shotgun proteomic data derived from bone collagen from modern, archaeological and extinct taxa. We further demonstrate how this method may be used to differentiate between modern contaminants and authentic ancient proteins using a case study from Neolithic dental calculus.",
keywords = "Authentication, Bioinformatics, Contamination, Palaeoproteomics",
author = "Abigail Rams{\o}e and {van Heekeren}, Vivian and Paola Ponce and Roman Fischer and Ian Barnes and Camilla Speller and Collins, {Matthew J.}",
year = "2020",
month = "3",
doi = "10.1016/j.jas.2020.105080",
language = "English",
volume = "115",
journal = "Journal of Archaeological Science",
issn = "0305-4403",
publisher = "Academic Press",

}

RIS

TY - JOUR

T1 - DeamiDATE 1.0

T2 - Site-specific deamidation as a tool to assess authenticity of members of ancient proteomes

AU - Ramsøe, Abigail

AU - van Heekeren, Vivian

AU - Ponce, Paola

AU - Fischer, Roman

AU - Barnes, Ian

AU - Speller, Camilla

AU - Collins, Matthew J.

PY - 2020/3

Y1 - 2020/3

N2 - Contamination is a potential problem in the study of ancient proteins, either from prior handling of the sample, laboratory consumables, or cross-sample carryover from mass spectrometers. Recently, deamidation of glutamine has been proposed as a measure for assessing the degradation of ancient proteins. Here, we present deamiDATE 1.0, a method for the authentication of ancient proteins using measure of site-specific deamidation rates. We test this approach on shotgun proteomic data derived from bone collagen from modern, archaeological and extinct taxa. We further demonstrate how this method may be used to differentiate between modern contaminants and authentic ancient proteins using a case study from Neolithic dental calculus.

AB - Contamination is a potential problem in the study of ancient proteins, either from prior handling of the sample, laboratory consumables, or cross-sample carryover from mass spectrometers. Recently, deamidation of glutamine has been proposed as a measure for assessing the degradation of ancient proteins. Here, we present deamiDATE 1.0, a method for the authentication of ancient proteins using measure of site-specific deamidation rates. We test this approach on shotgun proteomic data derived from bone collagen from modern, archaeological and extinct taxa. We further demonstrate how this method may be used to differentiate between modern contaminants and authentic ancient proteins using a case study from Neolithic dental calculus.

KW - Authentication

KW - Bioinformatics

KW - Contamination

KW - Palaeoproteomics

U2 - 10.1016/j.jas.2020.105080

DO - 10.1016/j.jas.2020.105080

M3 - Journal article

AN - SCOPUS:85078191602

VL - 115

JO - Journal of Archaeological Science

JF - Journal of Archaeological Science

SN - 0305-4403

M1 - 105080

ER -

ID: 241988791