Use of emerging complete organelle DNA reference database in the diet analysis of the herbivorous wood grouse, western capercaillies (Tetrao urogallus)

Research output: Contribution to conferencePosterResearch

Standard

Use of emerging complete organelle DNA reference database in the diet analysis of the herbivorous wood grouse, western capercaillies (Tetrao urogallus). / Chua, Physilia; Boessenkool, Sanne; Alsos, Inger Greve; Ekrem, Torbjørn; Crampton-Platt, Alex; Bohmann, Kristine.

2019. Poster session presented at 8th International Barcode of Life Conference 2019, Trondheim, Norway.

Research output: Contribution to conferencePosterResearch

Harvard

Chua, P, Boessenkool, S, Alsos, IG, Ekrem, T, Crampton-Platt, A & Bohmann, K 2019, 'Use of emerging complete organelle DNA reference database in the diet analysis of the herbivorous wood grouse, western capercaillies (Tetrao urogallus)', 8th International Barcode of Life Conference 2019, Trondheim, Norway, 17/06/2019 - 20/06/2019. <https://www.plantid.uio.no/research/research-output/posters/190618-physilia.html>

APA

Chua, P., Boessenkool, S., Alsos, I. G., Ekrem, T., Crampton-Platt, A., & Bohmann, K. (2019). Use of emerging complete organelle DNA reference database in the diet analysis of the herbivorous wood grouse, western capercaillies (Tetrao urogallus). Poster session presented at 8th International Barcode of Life Conference 2019, Trondheim, Norway. https://www.plantid.uio.no/research/research-output/posters/190618-physilia.html

Vancouver

Chua P, Boessenkool S, Alsos IG, Ekrem T, Crampton-Platt A, Bohmann K. Use of emerging complete organelle DNA reference database in the diet analysis of the herbivorous wood grouse, western capercaillies (Tetrao urogallus). 2019. Poster session presented at 8th International Barcode of Life Conference 2019, Trondheim, Norway.

Author

Chua, Physilia ; Boessenkool, Sanne ; Alsos, Inger Greve ; Ekrem, Torbjørn ; Crampton-Platt, Alex ; Bohmann, Kristine. / Use of emerging complete organelle DNA reference database in the diet analysis of the herbivorous wood grouse, western capercaillies (Tetrao urogallus). Poster session presented at 8th International Barcode of Life Conference 2019, Trondheim, Norway.

Bibtex

@conference{60e22143c9ca4ada9cbdcabc941ea4f2,
title = "Use of emerging complete organelle DNA reference database in the diet analysis of the herbivorous wood grouse, western capercaillies (Tetrao urogallus)",
abstract = "Diet analysis is an important tool in conservation biology, where it can provide information on species{\textquoteright} resource use and habitat requirements. Environmental DNA (eDNA) is a valuable source for diet reconstruction, but the use of reference databases comprising short DNA sequence markers can limit the resolution for accurate species identification. We explore how the optimal use of emerging complete organelle DNA reference databases such as PhyloNorway, may lead to better taxonomic identification of plants in animal diet. We collected faecal samples from eight individual westerncapercaillies located in Norway. We used shotgun sequencing to obtain 150bp paired reads and diet information was retrieved by blasting to both GenBank and the PhyloNorway reference database. Our preliminary results suggest that when using PhyloNorway as a reference database, we were able to retrieve at least 160 times more reads assigned to the species-level.",
author = "Physilia Chua and Sanne Boessenkool and Alsos, {Inger Greve} and Torbj{\o}rn Ekrem and Alex Crampton-Platt and Kristine Bohmann",
year = "2019",
language = "English",
note = "8th International Barcode of Life Conference 2019, iBOL2019 ; Conference date: 17-06-2019 Through 20-06-2019",
url = "http://dnabarcodes2019.org/",

}

RIS

TY - CONF

T1 - Use of emerging complete organelle DNA reference database in the diet analysis of the herbivorous wood grouse, western capercaillies (Tetrao urogallus)

AU - Chua, Physilia

AU - Boessenkool, Sanne

AU - Alsos, Inger Greve

AU - Ekrem, Torbjørn

AU - Crampton-Platt, Alex

AU - Bohmann, Kristine

PY - 2019

Y1 - 2019

N2 - Diet analysis is an important tool in conservation biology, where it can provide information on species’ resource use and habitat requirements. Environmental DNA (eDNA) is a valuable source for diet reconstruction, but the use of reference databases comprising short DNA sequence markers can limit the resolution for accurate species identification. We explore how the optimal use of emerging complete organelle DNA reference databases such as PhyloNorway, may lead to better taxonomic identification of plants in animal diet. We collected faecal samples from eight individual westerncapercaillies located in Norway. We used shotgun sequencing to obtain 150bp paired reads and diet information was retrieved by blasting to both GenBank and the PhyloNorway reference database. Our preliminary results suggest that when using PhyloNorway as a reference database, we were able to retrieve at least 160 times more reads assigned to the species-level.

AB - Diet analysis is an important tool in conservation biology, where it can provide information on species’ resource use and habitat requirements. Environmental DNA (eDNA) is a valuable source for diet reconstruction, but the use of reference databases comprising short DNA sequence markers can limit the resolution for accurate species identification. We explore how the optimal use of emerging complete organelle DNA reference databases such as PhyloNorway, may lead to better taxonomic identification of plants in animal diet. We collected faecal samples from eight individual westerncapercaillies located in Norway. We used shotgun sequencing to obtain 150bp paired reads and diet information was retrieved by blasting to both GenBank and the PhyloNorway reference database. Our preliminary results suggest that when using PhyloNorway as a reference database, we were able to retrieve at least 160 times more reads assigned to the species-level.

M3 - Poster

T2 - 8th International Barcode of Life Conference 2019

Y2 - 17 June 2019 through 20 June 2019

ER -

ID: 227704200