Use of emerging complete organelle DNA reference database in the diet analysis of the herbivorous wood grouse, western capercaillies (Tetrao urogallus)

Research output: Contribution to conferencePosterResearch

  • Physilia Chua
  • Sanne Boessenkool
  • Inger Greve Alsos
  • Torbjørn Ekrem
  • Alex Crampton-Platt
  • Bohmann, Kristine
Diet analysis is an important tool in conservation biology, where it can provide information on species’ resource use and habitat requirements. Environmental DNA (eDNA) is a valuable source for diet reconstruction, but the use of reference databases comprising short DNA sequence markers can limit the resolution for accurate species identification. We explore how the optimal use of emerging complete organelle DNA reference databases such as PhyloNorway, may lead to better taxonomic identification of plants in animal diet. We collected faecal samples from eight individual western
capercaillies located in Norway. We used shotgun sequencing to obtain 150bp paired reads and diet information was retrieved by blasting to both GenBank and the PhyloNorway reference database. Our preliminary results suggest that when using PhyloNorway as a reference database, we were able to retrieve at least 160 times more reads assigned to the species-level.
Original languageEnglish
Publication date2019
Publication statusPublished - 2019
Event8th International Barcode of Life Conference 2019 - Trondheim, Norway, Trondheim, Norway
Duration: 17 Jun 201920 Jun 2019
http://dnabarcodes2019.org/

Conference

Conference8th International Barcode of Life Conference 2019
LocationTrondheim, Norway
CountryNorway
CityTrondheim
Period17/06/201920/06/2019
Internet address

ID: 227704200