A simplified bacterial community found within the epidermis than at the epidermal surface of atopic dermatitis patients and healthy controls
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A simplified bacterial community found within the epidermis than at the epidermal surface of atopic dermatitis patients and healthy controls. / Barnes, Christopher J.; Asplund, Maria; Clausen, Maja-Lisa; Rasmussen, Linett; Olesen, Caroline Meyer; Yüksel, Yasemin Topal; Andersen, Paal Skytt; Litman, Thomas; Holmstrøm, Kim; Bay, Lene; Fritz, Blaine Gabriel; Bjarnsholt, Thomas; Agner, Tove; Hansen, Anders Johannes.
In: BMC Microbiology, Vol. 23, 273, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - A simplified bacterial community found within the epidermis than at the epidermal surface of atopic dermatitis patients and healthy controls
AU - Barnes, Christopher J.
AU - Asplund, Maria
AU - Clausen, Maja-Lisa
AU - Rasmussen, Linett
AU - Olesen, Caroline Meyer
AU - Yüksel, Yasemin Topal
AU - Andersen, Paal Skytt
AU - Litman, Thomas
AU - Holmstrøm, Kim
AU - Bay, Lene
AU - Fritz, Blaine Gabriel
AU - Bjarnsholt, Thomas
AU - Agner, Tove
AU - Hansen, Anders Johannes
N1 - Publisher Copyright: © 2023, BioMed Central Ltd., part of Springer Nature.
PY - 2023
Y1 - 2023
N2 - There has been considerable research into the understanding of the healthy skin microbiome. Similarly, there is also a considerable body of research into whether specific microbes contribute to skin disorders, with atopic dermatitis (AD) routinely linked to increased Staphylococcus aureus (S. aureus) colonisation. In this study, the epidermal surface of participants was sampled using swabs, while serial tape-stripping (35 tapes) was performed to sample through the stratum corneum. Samples were taken from AD patients and healthy controls, and the bacterial communities were profiled by metabarcoding the universal V3-V4 16S rRNA region. Results show that the majority of bacterial richness is located within the outermost layers of the stratum corneum, however there were many taxa that were found almost exclusively at the very outermost layer of the epidermis. We therefore hypothesise that tape-stripping can be performed to investigate the ‘core microbiome’ of participants by removing environmental contaminants. Interestingly, significant community variation between AD patients and healthy controls was only observable at the epidermal surface, yet a number of individual taxa were found to consistently differ with AD status across the entire epidermis (i.e. both the epidermal surface and within the epidermis). Sampling strategy could therefore be tailored dependent on the hypothesis, with sampling for forensic applications best performed using surface swabs and outer tapes, while profiling sub-surface communities may better reflect host genome and immunological status.
AB - There has been considerable research into the understanding of the healthy skin microbiome. Similarly, there is also a considerable body of research into whether specific microbes contribute to skin disorders, with atopic dermatitis (AD) routinely linked to increased Staphylococcus aureus (S. aureus) colonisation. In this study, the epidermal surface of participants was sampled using swabs, while serial tape-stripping (35 tapes) was performed to sample through the stratum corneum. Samples were taken from AD patients and healthy controls, and the bacterial communities were profiled by metabarcoding the universal V3-V4 16S rRNA region. Results show that the majority of bacterial richness is located within the outermost layers of the stratum corneum, however there were many taxa that were found almost exclusively at the very outermost layer of the epidermis. We therefore hypothesise that tape-stripping can be performed to investigate the ‘core microbiome’ of participants by removing environmental contaminants. Interestingly, significant community variation between AD patients and healthy controls was only observable at the epidermal surface, yet a number of individual taxa were found to consistently differ with AD status across the entire epidermis (i.e. both the epidermal surface and within the epidermis). Sampling strategy could therefore be tailored dependent on the hypothesis, with sampling for forensic applications best performed using surface swabs and outer tapes, while profiling sub-surface communities may better reflect host genome and immunological status.
KW - Atopic dermatitis
KW - Bacteria
KW - Epidermis
KW - Fluorescent in situ hybridisation
KW - Skin microbiome
KW - Staphylococcus aureus
U2 - 10.1186/s12866-023-03012-7
DO - 10.1186/s12866-023-03012-7
M3 - Journal article
C2 - 37773096
AN - SCOPUS:85173566123
VL - 23
JO - BMC Microbiology
JF - BMC Microbiology
SN - 1471-2180
M1 - 273
ER -
ID: 369974771