Analysing the effects of distance, taxon and biomass on vertebrate detections using bulk-collected carrion fly iDNA
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Analysing the effects of distance, taxon and biomass on vertebrate detections using bulk-collected carrion fly iDNA. / Fernandes, Kristen; Bateman, Philip W.; Saunders, Benjamin J.; Gibberd, Mark; Bunce, Michael; Bohmann, Kristine; Nevill, Paul.
In: Royal Society Open Science, Vol. 11, No. 4, 231286, 2024.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Analysing the effects of distance, taxon and biomass on vertebrate detections using bulk-collected carrion fly iDNA
AU - Fernandes, Kristen
AU - Bateman, Philip W.
AU - Saunders, Benjamin J.
AU - Gibberd, Mark
AU - Bunce, Michael
AU - Bohmann, Kristine
AU - Nevill, Paul
N1 - Publisher Copyright: © 2024 The Authors.
PY - 2024
Y1 - 2024
N2 - Invertebrate-derived DNA (iDNA) metabarcoding from carrion flies is a powerful, non-invasive tool that has value for assessing vertebrate diversity. However, unknowns exist around the factors that influence vertebrate detections, such as spatial limits to iDNA signals or if detections are influenced by taxonomic class or estimated biomass of the vertebrates of interest. Using a bulk-collection method, we captured flies from within a zoo and along transects extending 4 km away from this location. From 920 flies, we detected 28 vertebrate species. Of the 28 detected species, we identified 9 species kept at the zoo, 8 mammals and 1 bird, but no reptiles. iDNA detections were highly geographically localized, and only a few zoo animals were detected outside the zoo setting. However, due to the low number of detections in our dataset, we found no influence of the taxonomic group or the estimated biomass of animals on their detectability. Our data suggest that iDNA detections from bulk-collected carrion flies, at least in urban settings in Australia, are predominantly determined by geographic proximity to the sampling location. This study presents an important step in understanding how iDNA techniques can be used in biodiversity monitoring.
AB - Invertebrate-derived DNA (iDNA) metabarcoding from carrion flies is a powerful, non-invasive tool that has value for assessing vertebrate diversity. However, unknowns exist around the factors that influence vertebrate detections, such as spatial limits to iDNA signals or if detections are influenced by taxonomic class or estimated biomass of the vertebrates of interest. Using a bulk-collection method, we captured flies from within a zoo and along transects extending 4 km away from this location. From 920 flies, we detected 28 vertebrate species. Of the 28 detected species, we identified 9 species kept at the zoo, 8 mammals and 1 bird, but no reptiles. iDNA detections were highly geographically localized, and only a few zoo animals were detected outside the zoo setting. However, due to the low number of detections in our dataset, we found no influence of the taxonomic group or the estimated biomass of animals on their detectability. Our data suggest that iDNA detections from bulk-collected carrion flies, at least in urban settings in Australia, are predominantly determined by geographic proximity to the sampling location. This study presents an important step in understanding how iDNA techniques can be used in biodiversity monitoring.
KW - biomonitoring
KW - invertebrate-derived DNA
KW - metabarcoding
KW - terrestrial vertebrate
U2 - 10.1098/rsos.231286
DO - 10.1098/rsos.231286
M3 - Journal article
C2 - 38577218
AN - SCOPUS:85189888524
VL - 11
JO - Royal Society Open Science
JF - Royal Society Open Science
SN - 2054-5703
IS - 4
M1 - 231286
ER -
ID: 389666753