Integration of fluorescence in situ hybridization and chromosome-length genome assemblies revealed synteny map for guinea pig, naked mole-rat, and human

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Integration of fluorescence in situ hybridization and chromosome-length genome assemblies revealed synteny map for guinea pig, naked mole-rat, and human. / Romanenko, Svetlana A.; Kliver, Sergei F.; Serdyukova, Natalia A.; Perelman, Polina L.; Trifonov, Vladimir A.; Seluanov, Andrei; Gorbunova, Vera; Azpurua, Jorge; Pereira, Jorge C.; Ferguson-Smith, Malcolm A.; Graphodatsky, Alexander S.

In: Scientific Reports, Vol. 13, 21055, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Romanenko, SA, Kliver, SF, Serdyukova, NA, Perelman, PL, Trifonov, VA, Seluanov, A, Gorbunova, V, Azpurua, J, Pereira, JC, Ferguson-Smith, MA & Graphodatsky, AS 2023, 'Integration of fluorescence in situ hybridization and chromosome-length genome assemblies revealed synteny map for guinea pig, naked mole-rat, and human', Scientific Reports, vol. 13, 21055. https://doi.org/10.1038/s41598-023-46595-x

APA

Romanenko, S. A., Kliver, S. F., Serdyukova, N. A., Perelman, P. L., Trifonov, V. A., Seluanov, A., Gorbunova, V., Azpurua, J., Pereira, J. C., Ferguson-Smith, M. A., & Graphodatsky, A. S. (2023). Integration of fluorescence in situ hybridization and chromosome-length genome assemblies revealed synteny map for guinea pig, naked mole-rat, and human. Scientific Reports, 13, [21055]. https://doi.org/10.1038/s41598-023-46595-x

Vancouver

Romanenko SA, Kliver SF, Serdyukova NA, Perelman PL, Trifonov VA, Seluanov A et al. Integration of fluorescence in situ hybridization and chromosome-length genome assemblies revealed synteny map for guinea pig, naked mole-rat, and human. Scientific Reports. 2023;13. 21055. https://doi.org/10.1038/s41598-023-46595-x

Author

Romanenko, Svetlana A. ; Kliver, Sergei F. ; Serdyukova, Natalia A. ; Perelman, Polina L. ; Trifonov, Vladimir A. ; Seluanov, Andrei ; Gorbunova, Vera ; Azpurua, Jorge ; Pereira, Jorge C. ; Ferguson-Smith, Malcolm A. ; Graphodatsky, Alexander S. / Integration of fluorescence in situ hybridization and chromosome-length genome assemblies revealed synteny map for guinea pig, naked mole-rat, and human. In: Scientific Reports. 2023 ; Vol. 13.

Bibtex

@article{444132c93aa14d5fb362d1de2fc98b8e,
title = "Integration of fluorescence in situ hybridization and chromosome-length genome assemblies revealed synteny map for guinea pig, naked mole-rat, and human",
abstract = "Descriptions of karyotypes of many animal species are currently available. In addition, there has been a significant increase in the number of sequenced genomes and an ever-improving quality of genome assembly. To close the gap between genomic and cytogenetic data we applied fluorescent in situ hybridization (FISH) and Hi-C technology to make the first full chromosome-level genome comparison of the guinea pig (Cavia porcellus), naked mole-rat (Heterocephalus glaber), and human. Comparative chromosome maps obtained by FISH with chromosome-specific probes link genomic scaffolds to individual chromosomes and orient them relative to centromeres and heterochromatic blocks. Hi-C assembly made it possible to close all gaps on the comparative maps and to reveal additional rearrangements that distinguish the karyotypes of the three species. As a result, we integrated the bioinformatic and cytogenetic data and adjusted the previous comparative maps and genome assemblies of the guinea pig, naked mole-rat, and human. Syntenic associations in the two hystricomorphs indicate features of their putative ancestral karyotype. We postulate that the two approaches applied in this study complement one another and provide complete information about the organization of these genomes at the chromosome level.",
author = "Romanenko, {Svetlana A.} and Kliver, {Sergei F.} and Serdyukova, {Natalia A.} and Perelman, {Polina L.} and Trifonov, {Vladimir A.} and Andrei Seluanov and Vera Gorbunova and Jorge Azpurua and Pereira, {Jorge C.} and Ferguson-Smith, {Malcolm A.} and Graphodatsky, {Alexander S.}",
note = "Publisher Copyright: {\textcopyright} 2023, The Author(s).",
year = "2023",
doi = "10.1038/s41598-023-46595-x",
language = "English",
volume = "13",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Integration of fluorescence in situ hybridization and chromosome-length genome assemblies revealed synteny map for guinea pig, naked mole-rat, and human

AU - Romanenko, Svetlana A.

AU - Kliver, Sergei F.

AU - Serdyukova, Natalia A.

AU - Perelman, Polina L.

AU - Trifonov, Vladimir A.

AU - Seluanov, Andrei

AU - Gorbunova, Vera

AU - Azpurua, Jorge

AU - Pereira, Jorge C.

AU - Ferguson-Smith, Malcolm A.

AU - Graphodatsky, Alexander S.

N1 - Publisher Copyright: © 2023, The Author(s).

PY - 2023

Y1 - 2023

N2 - Descriptions of karyotypes of many animal species are currently available. In addition, there has been a significant increase in the number of sequenced genomes and an ever-improving quality of genome assembly. To close the gap between genomic and cytogenetic data we applied fluorescent in situ hybridization (FISH) and Hi-C technology to make the first full chromosome-level genome comparison of the guinea pig (Cavia porcellus), naked mole-rat (Heterocephalus glaber), and human. Comparative chromosome maps obtained by FISH with chromosome-specific probes link genomic scaffolds to individual chromosomes and orient them relative to centromeres and heterochromatic blocks. Hi-C assembly made it possible to close all gaps on the comparative maps and to reveal additional rearrangements that distinguish the karyotypes of the three species. As a result, we integrated the bioinformatic and cytogenetic data and adjusted the previous comparative maps and genome assemblies of the guinea pig, naked mole-rat, and human. Syntenic associations in the two hystricomorphs indicate features of their putative ancestral karyotype. We postulate that the two approaches applied in this study complement one another and provide complete information about the organization of these genomes at the chromosome level.

AB - Descriptions of karyotypes of many animal species are currently available. In addition, there has been a significant increase in the number of sequenced genomes and an ever-improving quality of genome assembly. To close the gap between genomic and cytogenetic data we applied fluorescent in situ hybridization (FISH) and Hi-C technology to make the first full chromosome-level genome comparison of the guinea pig (Cavia porcellus), naked mole-rat (Heterocephalus glaber), and human. Comparative chromosome maps obtained by FISH with chromosome-specific probes link genomic scaffolds to individual chromosomes and orient them relative to centromeres and heterochromatic blocks. Hi-C assembly made it possible to close all gaps on the comparative maps and to reveal additional rearrangements that distinguish the karyotypes of the three species. As a result, we integrated the bioinformatic and cytogenetic data and adjusted the previous comparative maps and genome assemblies of the guinea pig, naked mole-rat, and human. Syntenic associations in the two hystricomorphs indicate features of their putative ancestral karyotype. We postulate that the two approaches applied in this study complement one another and provide complete information about the organization of these genomes at the chromosome level.

U2 - 10.1038/s41598-023-46595-x

DO - 10.1038/s41598-023-46595-x

M3 - Journal article

C2 - 38030702

AN - SCOPUS:85178234775

VL - 13

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 21055

ER -

ID: 376450427