Long-term evolution of antibiotic tolerance in Pseudomonas aeruginosa lung  infections

Research output: Contribution to journal › Journal article › Research › peer-review

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Long-term evolution of antibiotic tolerance in Pseudomonas aeruginosa lung  infections. / Ghoul, Melanie; Andersen, Sandra B.; Marvig, Rasmus L.; Johansen, Helle K.; Jelsbak, Lars; Molin, Søren; Perron, Gabriel; Griffin, Ashleigh S.

In: Evolution Letters, Vol. 7, No. 6, 2023, p. 389–400.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ghoul, M, Andersen, SB, Marvig, RL, Johansen, HK, Jelsbak, L, Molin, S, Perron, G & Griffin, AS 2023, 'Long-term evolution of antibiotic tolerance in Pseudomonas aeruginosa lung  infections', Evolution Letters, vol. 7, no. 6, pp. 389–400. https://doi.org/10.1093/evlett/qrad034

APA

Ghoul, M., Andersen, S. B., Marvig, R. L., Johansen, H. K., Jelsbak, L., Molin, S., Perron, G., & Griffin, A. S. (2023). Long-term evolution of antibiotic tolerance in Pseudomonas aeruginosa lung  infections. Evolution Letters, 7(6), 389–400. https://doi.org/10.1093/evlett/qrad034

Vancouver

Ghoul M, Andersen SB, Marvig RL, Johansen HK, Jelsbak L, Molin S et al. Long-term evolution of antibiotic tolerance in Pseudomonas aeruginosa lung  infections. Evolution Letters. 2023;7(6):389–400. https://doi.org/10.1093/evlett/qrad034

Author

Ghoul, Melanie ; Andersen, Sandra B. ; Marvig, Rasmus L. ; Johansen, Helle K. ; Jelsbak, Lars ; Molin, Søren ; Perron, Gabriel ; Griffin, Ashleigh S. / Long-term evolution of antibiotic tolerance in Pseudomonas aeruginosa lung  infections. In: Evolution Letters. 2023 ; Vol. 7, No. 6. pp. 389–400.

Bibtex

@article{5a82a5077e0242928ccc0c9adf6fec57,
title = "Long-term evolution of antibiotic tolerance in Pseudomonas aeruginosa lung  infections",
abstract = "Pathogenic bacteria respond to antibiotic pressure with the evolution of resistance but survival can also depend on their ability to tolerate antibiotic treatment, known as tolerance. While a variety of resistance mechanisms and underlying genetics are well characterized in vitro and in vivo, an understanding of the evolution of tolerance, and how it interacts with resistance in situ is lacking. We assayed for tolerance and resistance in isolates of Pseudomonas aeruginosa from chronic cystic fibrosis lung infections spanning up to 40 years of evolution, with 3 clinically relevant antibiotics: meropenem, ciprofloxacin, and tobramycin. We present evidence that tolerance is under positive selection in the lung and that it can act as an evolutionary stepping stone to resistance. However, by examining evolutionary patterns across multiple patients in different clone types, a key result is that the potential for an association between the evolution of resistance and tolerance is not inevitable, and difficult to predict.",
author = "Melanie Ghoul and Andersen, {Sandra B.} and Marvig, {Rasmus L.} and Johansen, {Helle K.} and Lars Jelsbak and S{\o}ren Molin and Gabriel Perron and Griffin, {Ashleigh S.}",
year = "2023",
doi = "10.1093/evlett/qrad034",
language = "English",
volume = "7",
pages = "389–400",
journal = "Evolution Letters",
issn = "2056-3744",
publisher = "Wiley",
number = "6",

}

RIS

TY - JOUR

T1 - Long-term evolution of antibiotic tolerance in Pseudomonas aeruginosa lung  infections

AU - Ghoul, Melanie

AU - Andersen, Sandra B.

AU - Marvig, Rasmus L.

AU - Johansen, Helle K.

AU - Jelsbak, Lars

AU - Molin, Søren

AU - Perron, Gabriel

AU - Griffin, Ashleigh S.

PY - 2023

Y1 - 2023

N2 - Pathogenic bacteria respond to antibiotic pressure with the evolution of resistance but survival can also depend on their ability to tolerate antibiotic treatment, known as tolerance. While a variety of resistance mechanisms and underlying genetics are well characterized in vitro and in vivo, an understanding of the evolution of tolerance, and how it interacts with resistance in situ is lacking. We assayed for tolerance and resistance in isolates of Pseudomonas aeruginosa from chronic cystic fibrosis lung infections spanning up to 40 years of evolution, with 3 clinically relevant antibiotics: meropenem, ciprofloxacin, and tobramycin. We present evidence that tolerance is under positive selection in the lung and that it can act as an evolutionary stepping stone to resistance. However, by examining evolutionary patterns across multiple patients in different clone types, a key result is that the potential for an association between the evolution of resistance and tolerance is not inevitable, and difficult to predict.

AB - Pathogenic bacteria respond to antibiotic pressure with the evolution of resistance but survival can also depend on their ability to tolerate antibiotic treatment, known as tolerance. While a variety of resistance mechanisms and underlying genetics are well characterized in vitro and in vivo, an understanding of the evolution of tolerance, and how it interacts with resistance in situ is lacking. We assayed for tolerance and resistance in isolates of Pseudomonas aeruginosa from chronic cystic fibrosis lung infections spanning up to 40 years of evolution, with 3 clinically relevant antibiotics: meropenem, ciprofloxacin, and tobramycin. We present evidence that tolerance is under positive selection in the lung and that it can act as an evolutionary stepping stone to resistance. However, by examining evolutionary patterns across multiple patients in different clone types, a key result is that the potential for an association between the evolution of resistance and tolerance is not inevitable, and difficult to predict.

U2 - 10.1093/evlett/qrad034

DO - 10.1093/evlett/qrad034

M3 - Journal article

C2 - 38045720

VL - 7

SP - 389

EP - 400

JO - Evolution Letters

JF - Evolution Letters

SN - 2056-3744

IS - 6

ER -

ID: 369551102