TY - CHAP

T1 - Marine environmental DNA

T2 - Approaches, applications, and opportunities

AU - Eble, Jeff A.

AU - Daly-Engel, Toby S.

AU - DiBattista, Joseph D.

AU - Koziol, Adam

AU - Gaither, Michelle R.

PY - 2020

Y1 - 2020

N2 - Environmental DNA (eDNA) is increasingly being used to document species distributions and habitat use in marine systems, with much of the recent effort focused on leveraging advances in next-generation DNA sequencing to assess and track biodiversity across taxonomic groups. Environmental DNA offers a number of important advantages over traditional survey techniques, including non-invasive sampling, sampling where traditional approaches are impractical or inefficient (e.g. deep oceans), reduced cost, and increased detection sensitivity. However, eDNA applications are currently limited because of an insufficient understanding of the influence of sample source, analytical approach, and marker type on eDNA detections. Because approaches vary considerably among eDNA studies, we present a summary of the current state of the field and emerging best practices. The impact of observed variation in rates of eDNA production, persistence, and transport are also discussed and future research needs are highlighted with the goal of expanding eDNA applications, including the development of statistical models to improve the predictability of eDNA detection and quantification.

AB - Environmental DNA (eDNA) is increasingly being used to document species distributions and habitat use in marine systems, with much of the recent effort focused on leveraging advances in next-generation DNA sequencing to assess and track biodiversity across taxonomic groups. Environmental DNA offers a number of important advantages over traditional survey techniques, including non-invasive sampling, sampling where traditional approaches are impractical or inefficient (e.g. deep oceans), reduced cost, and increased detection sensitivity. However, eDNA applications are currently limited because of an insufficient understanding of the influence of sample source, analytical approach, and marker type on eDNA detections. Because approaches vary considerably among eDNA studies, we present a summary of the current state of the field and emerging best practices. The impact of observed variation in rates of eDNA production, persistence, and transport are also discussed and future research needs are highlighted with the goal of expanding eDNA applications, including the development of statistical models to improve the predictability of eDNA detection and quantification.

KW - TAXONOMIC RESOLUTION

KW - EXTRACELLULAR DNA

KW - EUKARYOTIC DIVERSITY

KW - SPECIES DETECTION

KW - DECAY-RATES

KW - EDNA

KW - BIODIVERSITY

KW - QUANTIFICATION

KW - DEGRADATION

KW - PERSISTENCE

U2 - 10.1016/bs.amb.2020.01.001

DO - 10.1016/bs.amb.2020.01.001

M3 - Book chapter

C2 - 32600544

SN - 978-0-12-822478-6

T3 - Advances in Marine Biology

SP - 141

EP - 169

BT - Advances in Marine Biology

A2 - Sheppard, C

PB - Elsevier

ER -