Peptide analysis of tooth enamel - A sex estimation tool for archaeological, anthropological, or forensic research
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Peptide analysis of tooth enamel - A sex estimation tool for archaeological, anthropological, or forensic research. / Mikšík, Ivan; Morvan, Marine; Brůžek, Jaroslav.
In: Journal of Separation Science, Vol. 46, No. 15, e2300183, 2023.Research output: Contribution to journal › Review › Research › peer-review
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TY - JOUR
T1 - Peptide analysis of tooth enamel - A sex estimation tool for archaeological, anthropological, or forensic research
AU - Mikšík, Ivan
AU - Morvan, Marine
AU - Brůžek, Jaroslav
N1 - © 2023 Wiley-VCH GmbH.
PY - 2023
Y1 - 2023
N2 - Proteomics has become an attractive method to study human and animal material, biological profile, and origin as an alternative to DNA analysis. It is limited by DNA amplification in ancient samples and its contamination, high cost, and limited preservation of nuclear DNA. Currently, three approaches are available to estimate sex-osteology, genomics, or proteomics, but little is known about the relative reliability of these methods in applied settings. Proteomics provides a new, seemingly simple, and relatively non-expensive way of sex estimation without the risk of contamination. Proteins can be preserved in hard teeth tissue (enamel) for tens of thousands of years. It uses two sexually distinct forms of the protein amelogenin in tooth enamel detectable by liquid chromatography-mass spectrometry; the protein amelogenin Y isoform is present in enamel dental tissue only in males, while amelogenin isoform X can be found in both sexes. From the point of view of archaeological, anthropological, and forensic research and applications, the reduced destruction of the methods used is essential, as well as the minimum requirements for sample size.
AB - Proteomics has become an attractive method to study human and animal material, biological profile, and origin as an alternative to DNA analysis. It is limited by DNA amplification in ancient samples and its contamination, high cost, and limited preservation of nuclear DNA. Currently, three approaches are available to estimate sex-osteology, genomics, or proteomics, but little is known about the relative reliability of these methods in applied settings. Proteomics provides a new, seemingly simple, and relatively non-expensive way of sex estimation without the risk of contamination. Proteins can be preserved in hard teeth tissue (enamel) for tens of thousands of years. It uses two sexually distinct forms of the protein amelogenin in tooth enamel detectable by liquid chromatography-mass spectrometry; the protein amelogenin Y isoform is present in enamel dental tissue only in males, while amelogenin isoform X can be found in both sexes. From the point of view of archaeological, anthropological, and forensic research and applications, the reduced destruction of the methods used is essential, as well as the minimum requirements for sample size.
KW - Male
KW - Female
KW - Animals
KW - Humans
KW - Amelogenin/chemistry
KW - Reproducibility of Results
KW - Peptides/analysis
KW - DNA/analysis
KW - Protein Isoforms
KW - Dental Enamel/chemistry
U2 - 10.1002/jssc.202300183
DO - 10.1002/jssc.202300183
M3 - Review
C2 - 37232204
VL - 46
JO - HRC & CC, Journal of High Resolution Chromatography and Chromatography Communications
JF - HRC & CC, Journal of High Resolution Chromatography and Chromatography Communications
SN - 1615-9306
IS - 15
M1 - e2300183
ER -
ID: 389072752