RIP mutated ITS genes in populations of Ophiocordyceps sinensis and their implications for molecular systematics

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RIP mutated ITS genes in populations of Ophiocordyceps sinensis and their implications for molecular systematics. / Li, Yi; Jiang, Lan; Wang, Ke; Wu, Hai-Jun; Yang, Rui-Heng; Yan, Yu-Jing; Bushley, Kathryn E.; Hawksworth, David L.; Wu, Zujian; Yao, Yi-Jian.

In: IMA Fungus, Vol. 11, 18, 2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Li, Y, Jiang, L, Wang, K, Wu, H-J, Yang, R-H, Yan, Y-J, Bushley, KE, Hawksworth, DL, Wu, Z & Yao, Y-J 2020, 'RIP mutated ITS genes in populations of Ophiocordyceps sinensis and their implications for molecular systematics', IMA Fungus, vol. 11, 18. https://doi.org/10.1186/s43008-020-00040-0

APA

Li, Y., Jiang, L., Wang, K., Wu, H-J., Yang, R-H., Yan, Y-J., Bushley, K. E., Hawksworth, D. L., Wu, Z., & Yao, Y-J. (2020). RIP mutated ITS genes in populations of Ophiocordyceps sinensis and their implications for molecular systematics. IMA Fungus, 11, [18]. https://doi.org/10.1186/s43008-020-00040-0

Vancouver

Li Y, Jiang L, Wang K, Wu H-J, Yang R-H, Yan Y-J et al. RIP mutated ITS genes in populations of Ophiocordyceps sinensis and their implications for molecular systematics. IMA Fungus. 2020;11. 18. https://doi.org/10.1186/s43008-020-00040-0

Author

Li, Yi ; Jiang, Lan ; Wang, Ke ; Wu, Hai-Jun ; Yang, Rui-Heng ; Yan, Yu-Jing ; Bushley, Kathryn E. ; Hawksworth, David L. ; Wu, Zujian ; Yao, Yi-Jian. / RIP mutated ITS genes in populations of Ophiocordyceps sinensis and their implications for molecular systematics. In: IMA Fungus. 2020 ; Vol. 11.

Bibtex

@article{9ff58682596f4b79a643bb0670a6d66f,
title = "RIP mutated ITS genes in populations of Ophiocordyceps sinensis and their implications for molecular systematics",
abstract = "Different hypotheses have been proposed to interpret the observed unusual ITS (internal transcribed spacer) sequences inOphiocordyceps sinensis. The coexistence of diverged ITS paralogs in a single genome was previously shown by amplifying the ITS region from mono-ascospore isolates using specific primers designed for different ITS paralog groups. Among those paralogs, are AT-biased ITS sequences which were hypothesized to result from repeat-induced point mutation (RIP). This is a process that detects and mutates repetitive DNA and frequently leads to epigenetic silencing, and these mutations have been interpreted as pseudogenes. Here we investigate the occurrence and frequency of ITS pseudogenes in populations ofO. sinensisusing large-scale sampling, and discusses the implications of ITS pseudogenes for fungal phylogenetic and evolutionary studies. Our results demonstrate a wide distribution of ITS pseudogenes amongst different geographic populations, and indicate how ITS pseudogenes can contribute to the reconstruction of the evolutionary history of the species.",
keywords = "Caterpillar fungus, Cordyceps sinensis, ITS pseudogene, Haplotype, Phylogeny, INTERNAL TRANSCRIBED SPACER, INDUCED POINT MUTATION, RIBOSOMAL DNA, PHYLOGENETIC-RELATIONSHIPS, CATERPILLAR FUNGUS, EVOLUTION, SEQUENCE, NRDNA, GENOME, HETEROGENEITY",
author = "Yi Li and Lan Jiang and Ke Wang and Hai-Jun Wu and Rui-Heng Yang and Yu-Jing Yan and Bushley, {Kathryn E.} and Hawksworth, {David L.} and Zujian Wu and Yi-Jian Yao",
year = "2020",
doi = "10.1186/s43008-020-00040-0",
language = "English",
volume = "11",
journal = "I M A Fungus",
issn = "2210-6340",
publisher = "International Mycological Association",

}

RIS

TY - JOUR

T1 - RIP mutated ITS genes in populations of Ophiocordyceps sinensis and their implications for molecular systematics

AU - Li, Yi

AU - Jiang, Lan

AU - Wang, Ke

AU - Wu, Hai-Jun

AU - Yang, Rui-Heng

AU - Yan, Yu-Jing

AU - Bushley, Kathryn E.

AU - Hawksworth, David L.

AU - Wu, Zujian

AU - Yao, Yi-Jian

PY - 2020

Y1 - 2020

N2 - Different hypotheses have been proposed to interpret the observed unusual ITS (internal transcribed spacer) sequences inOphiocordyceps sinensis. The coexistence of diverged ITS paralogs in a single genome was previously shown by amplifying the ITS region from mono-ascospore isolates using specific primers designed for different ITS paralog groups. Among those paralogs, are AT-biased ITS sequences which were hypothesized to result from repeat-induced point mutation (RIP). This is a process that detects and mutates repetitive DNA and frequently leads to epigenetic silencing, and these mutations have been interpreted as pseudogenes. Here we investigate the occurrence and frequency of ITS pseudogenes in populations ofO. sinensisusing large-scale sampling, and discusses the implications of ITS pseudogenes for fungal phylogenetic and evolutionary studies. Our results demonstrate a wide distribution of ITS pseudogenes amongst different geographic populations, and indicate how ITS pseudogenes can contribute to the reconstruction of the evolutionary history of the species.

AB - Different hypotheses have been proposed to interpret the observed unusual ITS (internal transcribed spacer) sequences inOphiocordyceps sinensis. The coexistence of diverged ITS paralogs in a single genome was previously shown by amplifying the ITS region from mono-ascospore isolates using specific primers designed for different ITS paralog groups. Among those paralogs, are AT-biased ITS sequences which were hypothesized to result from repeat-induced point mutation (RIP). This is a process that detects and mutates repetitive DNA and frequently leads to epigenetic silencing, and these mutations have been interpreted as pseudogenes. Here we investigate the occurrence and frequency of ITS pseudogenes in populations ofO. sinensisusing large-scale sampling, and discusses the implications of ITS pseudogenes for fungal phylogenetic and evolutionary studies. Our results demonstrate a wide distribution of ITS pseudogenes amongst different geographic populations, and indicate how ITS pseudogenes can contribute to the reconstruction of the evolutionary history of the species.

KW - Caterpillar fungus

KW - Cordyceps sinensis

KW - ITS pseudogene

KW - Haplotype

KW - Phylogeny

KW - INTERNAL TRANSCRIBED SPACER

KW - INDUCED POINT MUTATION

KW - RIBOSOMAL DNA

KW - PHYLOGENETIC-RELATIONSHIPS

KW - CATERPILLAR FUNGUS

KW - EVOLUTION

KW - SEQUENCE

KW - NRDNA

KW - GENOME

KW - HETEROGENEITY

U2 - 10.1186/s43008-020-00040-0

DO - 10.1186/s43008-020-00040-0

M3 - Journal article

C2 - 32974122

VL - 11

JO - I M A Fungus

JF - I M A Fungus

SN - 2210-6340

M1 - 18

ER -

ID: 249909116