SARS-CoV-2 RNA stability in dry swabs for longer storage and transport at different temperatures
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SARS-CoV-2 RNA stability in dry swabs for longer storage and transport at different temperatures. / Alfaro-Núñez, Alonzo; Crone, Stephanie; Mortensen, Shila; Rosenstierne, Maiken Worsøe; Fomsgaard, Anders; Marving, Ellinor; Nielsen, Sofie Holdflod; Jørgensen, Michelle Grace Pinto; Polacek, Charlotta; Cohen, Arieh S.; Nielsen, Claus.
In: Transboundary and Emerging Diseases, Vol. 69, No. 2, 2022, p. 189-194.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - SARS-CoV-2 RNA stability in dry swabs for longer storage and transport at different temperatures
AU - Alfaro-Núñez, Alonzo
AU - Crone, Stephanie
AU - Mortensen, Shila
AU - Rosenstierne, Maiken Worsøe
AU - Fomsgaard, Anders
AU - Marving, Ellinor
AU - Nielsen, Sofie Holdflod
AU - Jørgensen, Michelle Grace Pinto
AU - Polacek, Charlotta
AU - Cohen, Arieh S.
AU - Nielsen, Claus
N1 - Publisher Copyright: © 2021 Wiley-VCH GmbH.
PY - 2022
Y1 - 2022
N2 - During the current COVID-19 pandemic, different methods have been used to evaluate patients with suspected SARS-CoV-2 infection. In this study, we experimentally evaluate the ability of spiked saliva-moist swabs and spiked swabs without any transport medium to retain SARS-CoV-2 for storage and transport at different environmental settings during different incubation time periods. Our results show that at ambient temperature of 20°C, SARS-CoV-2 RNA remains stable for up to 9 days allowing a long-time span for transport and storage without compromising clinical results. Additionally, this study demonstrates that saliva-moist swabs can also be stored at −20°C and +4°C for up to 26 days without affecting RT-qPCR results. Our data are relevant for low-and middle-income countries, which have limited access to rapid refrigerated transport and storage of samples representing an economical alternative. Finally, our study demonstrates the practical and economic advantage of using swabs without transport medium.
AB - During the current COVID-19 pandemic, different methods have been used to evaluate patients with suspected SARS-CoV-2 infection. In this study, we experimentally evaluate the ability of spiked saliva-moist swabs and spiked swabs without any transport medium to retain SARS-CoV-2 for storage and transport at different environmental settings during different incubation time periods. Our results show that at ambient temperature of 20°C, SARS-CoV-2 RNA remains stable for up to 9 days allowing a long-time span for transport and storage without compromising clinical results. Additionally, this study demonstrates that saliva-moist swabs can also be stored at −20°C and +4°C for up to 26 days without affecting RT-qPCR results. Our data are relevant for low-and middle-income countries, which have limited access to rapid refrigerated transport and storage of samples representing an economical alternative. Finally, our study demonstrates the practical and economic advantage of using swabs without transport medium.
KW - clinical samples
KW - COVID-19
KW - diagnostics
KW - oropharyngeal
KW - RNA quantification methods
KW - saliva
KW - surveillance
KW - transport medium
KW - virus stability
U2 - 10.1111/tbed.14339
DO - 10.1111/tbed.14339
M3 - Journal article
C2 - 34609793
AN - SCOPUS:85116779751
VL - 69
SP - 189
EP - 194
JO - Transboundary and Emerging Diseases
JF - Transboundary and Emerging Diseases
SN - 1865-1674
IS - 2
ER -
ID: 345317305