The effect of eraser sampling for proteomic analysis on Palaeolithic bone surface microtopography
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The effect of eraser sampling for proteomic analysis on Palaeolithic bone surface microtopography. / Sinet-Mathiot, Virginie; Martisius, Naomi L.; Schulz-Kornas, Ellen; van Casteren, Adam; Tsanova, Tsenka R.; Sirakov, Nikolay; Spasov, Rosen; Welker, Frido; Smith, Geoff M.; Hublin, Jean-Jacques.
In: Scientific Reports, Vol. 11, 23611, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The effect of eraser sampling for proteomic analysis on Palaeolithic bone surface microtopography
AU - Sinet-Mathiot, Virginie
AU - Martisius, Naomi L.
AU - Schulz-Kornas, Ellen
AU - van Casteren, Adam
AU - Tsanova, Tsenka R.
AU - Sirakov, Nikolay
AU - Spasov, Rosen
AU - Welker, Frido
AU - Smith, Geoff M.
AU - Hublin, Jean-Jacques
N1 - © 2021. The Author(s).
PY - 2021
Y1 - 2021
N2 - Bone surface modifications are crucial for understanding human subsistence and dietary behaviour, and can inform about the techniques employed in the production and use of bone tools. Permission to destructively sample such unique artefacts is not always granted. The recent development of non-destructive proteomic extraction techniques has provided some alternatives for the analysis of rare and culturally significant artefacts, including bone tools and personal ornaments. The Eraser Extraction Method (EEM), first developed for ZooMS analysis of parchment, has recently been applied to bone and ivory specimens. To test the potential impact of the EEM on ancient bone surfaces, we analyse six anthropogenically modified Palaeolithic bone specimens from Bacho Kiro Cave (Bulgaria) through a controlled sampling experiment using qualitative and 3D quantitative microscopy. Although the overall bone topography is generally preserved, our findings demonstrate a slight flattening of the microtopography alongside the formation of micro-striations associated with the use of the eraser for all bone specimens. Such modifications are similar to ancient use-wear traces. We therefore consider the EEM a destructive sampling approach for Palaeolithic bone surfaces. Together with low ZooMS success rates in some of the reported studies, the EEM might not be a suitable approach to taxonomically identify Pleistocene bone specimens.
AB - Bone surface modifications are crucial for understanding human subsistence and dietary behaviour, and can inform about the techniques employed in the production and use of bone tools. Permission to destructively sample such unique artefacts is not always granted. The recent development of non-destructive proteomic extraction techniques has provided some alternatives for the analysis of rare and culturally significant artefacts, including bone tools and personal ornaments. The Eraser Extraction Method (EEM), first developed for ZooMS analysis of parchment, has recently been applied to bone and ivory specimens. To test the potential impact of the EEM on ancient bone surfaces, we analyse six anthropogenically modified Palaeolithic bone specimens from Bacho Kiro Cave (Bulgaria) through a controlled sampling experiment using qualitative and 3D quantitative microscopy. Although the overall bone topography is generally preserved, our findings demonstrate a slight flattening of the microtopography alongside the formation of micro-striations associated with the use of the eraser for all bone specimens. Such modifications are similar to ancient use-wear traces. We therefore consider the EEM a destructive sampling approach for Palaeolithic bone surfaces. Together with low ZooMS success rates in some of the reported studies, the EEM might not be a suitable approach to taxonomically identify Pleistocene bone specimens.
U2 - 10.1038/s41598-021-02823-w
DO - 10.1038/s41598-021-02823-w
M3 - Journal article
C2 - 34880290
VL - 11
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 23611
ER -
ID: 287000810