The prevalence of controls in phyllosphere microbiome research: a methodological review

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Standard

The prevalence of controls in phyllosphere microbiome research : a methodological review. / Welsh, Brady L.; Eisenhofer, Raphael.

In: New Phytologist, Vol. 242, No. 1, 2024, p. 23-29.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Welsh, BL & Eisenhofer, R 2024, 'The prevalence of controls in phyllosphere microbiome research: a methodological review', New Phytologist, vol. 242, no. 1, pp. 23-29. https://doi.org/10.1111/nph.19573

APA

Welsh, B. L., & Eisenhofer, R. (2024). The prevalence of controls in phyllosphere microbiome research: a methodological review. New Phytologist, 242(1), 23-29. https://doi.org/10.1111/nph.19573

Vancouver

Welsh BL, Eisenhofer R. The prevalence of controls in phyllosphere microbiome research: a methodological review. New Phytologist. 2024;242(1):23-29. https://doi.org/10.1111/nph.19573

Author

Welsh, Brady L. ; Eisenhofer, Raphael. / The prevalence of controls in phyllosphere microbiome research : a methodological review. In: New Phytologist. 2024 ; Vol. 242, No. 1. pp. 23-29.

Bibtex

@article{a38efbac6173418aa721cf079ad6daec,
title = "The prevalence of controls in phyllosphere microbiome research: a methodological review",
abstract = "DNA contamination can critically confound microbiome studies. Here, we take a systematic approach to review the current literature and investigate the prevalence of contamination controls in phyllosphere microbiome research over the past decade. By utilising systematic review principles for this review, we were able to conduct a thorough investigation, screening 450 articles from three databases for eligibility and extracting data in a controlled and methodical manner. Worryingly, we observed a surprisingly low usage of both positive and negative contamination controls in phyllosphere research. As a result, we propose a set of minimum standards to combat the effects of contamination in future phyllosphere research.",
keywords = "contamination, controls, epiphytic, microbiome, microbiota, phyllosphere, plants, standards",
author = "Welsh, {Brady L.} and Raphael Eisenhofer",
note = "Publisher Copyright: {\textcopyright} 2024 The Authors. New Phytologist {\textcopyright} 2024 New Phytologist Foundation.",
year = "2024",
doi = "10.1111/nph.19573",
language = "English",
volume = "242",
pages = "23--29",
journal = "New Phytologist",
issn = "0028-646X",
publisher = "Academic Press",
number = "1",

}

RIS

TY - JOUR

T1 - The prevalence of controls in phyllosphere microbiome research

T2 - a methodological review

AU - Welsh, Brady L.

AU - Eisenhofer, Raphael

N1 - Publisher Copyright: © 2024 The Authors. New Phytologist © 2024 New Phytologist Foundation.

PY - 2024

Y1 - 2024

N2 - DNA contamination can critically confound microbiome studies. Here, we take a systematic approach to review the current literature and investigate the prevalence of contamination controls in phyllosphere microbiome research over the past decade. By utilising systematic review principles for this review, we were able to conduct a thorough investigation, screening 450 articles from three databases for eligibility and extracting data in a controlled and methodical manner. Worryingly, we observed a surprisingly low usage of both positive and negative contamination controls in phyllosphere research. As a result, we propose a set of minimum standards to combat the effects of contamination in future phyllosphere research.

AB - DNA contamination can critically confound microbiome studies. Here, we take a systematic approach to review the current literature and investigate the prevalence of contamination controls in phyllosphere microbiome research over the past decade. By utilising systematic review principles for this review, we were able to conduct a thorough investigation, screening 450 articles from three databases for eligibility and extracting data in a controlled and methodical manner. Worryingly, we observed a surprisingly low usage of both positive and negative contamination controls in phyllosphere research. As a result, we propose a set of minimum standards to combat the effects of contamination in future phyllosphere research.

KW - contamination

KW - controls

KW - epiphytic

KW - microbiome

KW - microbiota

KW - phyllosphere

KW - plants

KW - standards

U2 - 10.1111/nph.19573

DO - 10.1111/nph.19573

M3 - Journal article

C2 - 38339825

AN - SCOPUS:85184890168

VL - 242

SP - 23

EP - 29

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

IS - 1

ER -

ID: 383733910