The Tetragnatha kauaiensis Genome Sheds Light on the Origins of Genomic Novelty in Spiders
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
The Tetragnatha kauaiensis Genome Sheds Light on the Origins of Genomic Novelty in Spiders. / Cerca, José; Armstrong, Ellie E.; Vizueta, Joel; Fernández, Rosa; Dimitrov, Dimitar; Petersen, Bent; Prost, Stefan; Rozas, Julio; Petrov, Dmitri; Gillespie, Rosemary G.
In: Genome Biology and Evolution, Vol. 13, No. 12, evab262, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - The Tetragnatha kauaiensis Genome Sheds Light on the Origins of Genomic Novelty in Spiders
AU - Cerca, José
AU - Armstrong, Ellie E.
AU - Vizueta, Joel
AU - Fernández, Rosa
AU - Dimitrov, Dimitar
AU - Petersen, Bent
AU - Prost, Stefan
AU - Rozas, Julio
AU - Petrov, Dmitri
AU - Gillespie, Rosemary G.
N1 - © The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.
PY - 2021
Y1 - 2021
N2 - Spiders (Araneae) have a diverse spectrum of morphologies, behaviors, and physiologies. Attempts to understand the genomic-basis of this diversity are often hindered by their large, heterozygous, and AT-rich genomes with high repeat content resulting in highly fragmented, poor-quality assemblies. As a result, the key attributes of spider genomes, including gene family evolution, repeat content, and gene function, remain poorly understood. Here, we used Illumina and Dovetail Chicago technologies to sequence the genome of the long-jawed spider Tetragnatha kauaiensis, producing an assembly distributed along 3,925 scaffolds with an N50 of ∼2 Mb. Using comparative genomics tools, we explore genome evolution across available spider assemblies. Our findings suggest that the previously reported and vast genome size variation in spiders is linked to the different representation and number of transposable elements. Using statistical tools to uncover gene-family level evolution, we find expansions associated with the sensory perception of taste, immunity, and metabolism. In addition, we report strikingly different histories of chemosensory, venom, and silk gene families, with the first two evolving much earlier, affected by the ancestral whole genome duplication in Arachnopulmonata (∼450 Ma) and exhibiting higher numbers. Together, our findings reveal that spider genomes are highly variable and that genomic novelty may have been driven by the burst of an ancient whole genome duplication, followed by gene family and transposable element expansion.
AB - Spiders (Araneae) have a diverse spectrum of morphologies, behaviors, and physiologies. Attempts to understand the genomic-basis of this diversity are often hindered by their large, heterozygous, and AT-rich genomes with high repeat content resulting in highly fragmented, poor-quality assemblies. As a result, the key attributes of spider genomes, including gene family evolution, repeat content, and gene function, remain poorly understood. Here, we used Illumina and Dovetail Chicago technologies to sequence the genome of the long-jawed spider Tetragnatha kauaiensis, producing an assembly distributed along 3,925 scaffolds with an N50 of ∼2 Mb. Using comparative genomics tools, we explore genome evolution across available spider assemblies. Our findings suggest that the previously reported and vast genome size variation in spiders is linked to the different representation and number of transposable elements. Using statistical tools to uncover gene-family level evolution, we find expansions associated with the sensory perception of taste, immunity, and metabolism. In addition, we report strikingly different histories of chemosensory, venom, and silk gene families, with the first two evolving much earlier, affected by the ancestral whole genome duplication in Arachnopulmonata (∼450 Ma) and exhibiting higher numbers. Together, our findings reveal that spider genomes are highly variable and that genomic novelty may have been driven by the burst of an ancient whole genome duplication, followed by gene family and transposable element expansion.
U2 - 10.1093/gbe/evab262
DO - 10.1093/gbe/evab262
M3 - Journal article
C2 - 34849853
VL - 13
JO - Genome Biology and Evolution
JF - Genome Biology and Evolution
SN - 1759-6653
IS - 12
M1 - evab262
ER -
ID: 289397745