Analysis of chondroitin/dermatan sulphate disaccharides using high-performance liquid chromatography
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Analysis of chondroitin/dermatan sulphate disaccharides using high-performance liquid chromatography. / Mikšík, Ivan; Kubinová, Šárka; Morvan, Marine; Výborný, Karel; Tatar, Ameneh; Král, Vladimír; Záruba, Kamil; Sýkora, David.
In: Separations, Vol. 7, No. 3, 49, 09.2020, p. 1-7.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Analysis of chondroitin/dermatan sulphate disaccharides using high-performance liquid chromatography
AU - Mikšík, Ivan
AU - Kubinová, Šárka
AU - Morvan, Marine
AU - Výborný, Karel
AU - Tatar, Ameneh
AU - Král, Vladimír
AU - Záruba, Kamil
AU - Sýkora, David
N1 - Funding Information: Funding: This research was funded by the Czech Science Foundation, grant number 18-02597S, and SK and KV by Operational Programme Research, Development and Education in the framework of the project “Centre of Reconstructive Neuroscience” (registration number CZ.02.1.01/0.0./0.0/15_003/0000419). Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/9
Y1 - 2020/9
N2 - Chondroitin sulphates belong to a group of naturally occurring glycosaminoglycans and play a role in many physiological processes including ageing and the effects of various diseases. Research into chondroitin sulphates has found that the most important analytes are 4-and 6-sulphated disaccharides. We developed an HPLC method for the separation and quantification of underivatized chondroitin/dermatan sulphates—unsaturated disaccharides (4-and 6-sulphated disaccharides). This method is based on the separation of disaccharides by amido as well as amino columns under acidic conditions. These columns enabled the successful separation of 4-and 6-sulphated disaccharides using 50 (amido column) and 25 mmol/L (amino column) phosphate buffer, pH 4.25 (detection at 230 nm), at retention times of less than 10 min. The limit of quantification was 0.5 µg/mL. The applicability of this method was demonstrated through analysis of unsaturated disaccharides produced from the enzymatic digestion of chondroitin/dermatan sulphates of the solubilized extracellular matrix produced from porcine urinary bladder and human umbilical cord.
AB - Chondroitin sulphates belong to a group of naturally occurring glycosaminoglycans and play a role in many physiological processes including ageing and the effects of various diseases. Research into chondroitin sulphates has found that the most important analytes are 4-and 6-sulphated disaccharides. We developed an HPLC method for the separation and quantification of underivatized chondroitin/dermatan sulphates—unsaturated disaccharides (4-and 6-sulphated disaccharides). This method is based on the separation of disaccharides by amido as well as amino columns under acidic conditions. These columns enabled the successful separation of 4-and 6-sulphated disaccharides using 50 (amido column) and 25 mmol/L (amino column) phosphate buffer, pH 4.25 (detection at 230 nm), at retention times of less than 10 min. The limit of quantification was 0.5 µg/mL. The applicability of this method was demonstrated through analysis of unsaturated disaccharides produced from the enzymatic digestion of chondroitin/dermatan sulphates of the solubilized extracellular matrix produced from porcine urinary bladder and human umbilical cord.
KW - Chondroitin
KW - Dermatan sulphates
KW - Disaccharides
UR - http://www.scopus.com/inward/record.url?scp=85091258845&partnerID=8YFLogxK
U2 - 10.3390/separations7030049
DO - 10.3390/separations7030049
M3 - Journal article
AN - SCOPUS:85091258845
VL - 7
SP - 1
EP - 7
JO - Separations
JF - Separations
SN - 2297-8739
IS - 3
M1 - 49
ER -
ID: 389073421