Analysis of chondroitin/dermatan sulphate disaccharides using high-performance liquid chromatography
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Chondroitin sulphates belong to a group of naturally occurring glycosaminoglycans and play a role in many physiological processes including ageing and the effects of various diseases. Research into chondroitin sulphates has found that the most important analytes are 4-and 6-sulphated disaccharides. We developed an HPLC method for the separation and quantification of underivatized chondroitin/dermatan sulphates—unsaturated disaccharides (4-and 6-sulphated disaccharides). This method is based on the separation of disaccharides by amido as well as amino columns under acidic conditions. These columns enabled the successful separation of 4-and 6-sulphated disaccharides using 50 (amido column) and 25 mmol/L (amino column) phosphate buffer, pH 4.25 (detection at 230 nm), at retention times of less than 10 min. The limit of quantification was 0.5 µg/mL. The applicability of this method was demonstrated through analysis of unsaturated disaccharides produced from the enzymatic digestion of chondroitin/dermatan sulphates of the solubilized extracellular matrix produced from porcine urinary bladder and human umbilical cord.
Original language | English |
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Article number | 49 |
Journal | Separations |
Volume | 7 |
Issue number | 3 |
Pages (from-to) | 1-7 |
Number of pages | 7 |
DOIs | |
Publication status | Published - Sep 2020 |
Bibliographical note
Funding Information:
Funding: This research was funded by the Czech Science Foundation, grant number 18-02597S, and SK and KV by Operational Programme Research, Development and Education in the framework of the project “Centre of Reconstructive Neuroscience” (registration number CZ.02.1.01/0.0./0.0/15_003/0000419).
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
- Chondroitin, Dermatan sulphates, Disaccharides
Research areas
ID: 389073421