Modulation of the electroosmotic mobility using polyelectrolyte multilayer coatings for protein analysis by capillary electrophoresis
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Modulation of the electroosmotic mobility using polyelectrolyte multilayer coatings for protein analysis by capillary electrophoresis. / Leclercq, Laurent; Morvan, Marine; Koch, Jens; Neusüß, Christian; Cottet, Hervé.
In: Analytica Chimica Acta, Vol. 1057, 30.05.2019, p. 152-161.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Modulation of the electroosmotic mobility using polyelectrolyte multilayer coatings for protein analysis by capillary electrophoresis
AU - Leclercq, Laurent
AU - Morvan, Marine
AU - Koch, Jens
AU - Neusüß, Christian
AU - Cottet, Hervé
N1 - Publisher Copyright: © 2019 Elsevier B.V.
PY - 2019/5/30
Y1 - 2019/5/30
N2 - Successive multiple ionic-polymer layers (SMIL) coatings have been often used in capillary electrophoresis due to their simplicity to implement and regenerate. However, the performances of the separation are strongly dependent on the nature of the polyelectrolyte partners used to build the SMIL coating. In this work, we investigate new couples of polyelectrolytes that were not tested before: namely, polybrene (PB), quaternized diethylaminoethyl dextran (DEAEDq) and ε-poly(lysine) (ε-PLL), as polycations, in combination with poly(acrylic acid), dextran sulfate, poly(styrenesulfonate), poly(methacrylic acid) and poly(L-lysine citramide), as polyanions. Systematic study of intra- and inter-capillaries repeatabilities/reproducibilities was performed based on the determination of migration time, separation efficiency and electroosmotic mobility. Interestingly, the electroosmotic flow was found to vary with the nature of the polycation on a broad range of electroosmotic mobility decreasing in magnitude in the order of PB>ε-PLL > DEAEDq, whatever the polyanion associated. Application of the coatings to the separation of proteins is illustrated in a 0.5 M acetic acid BGE, including CE-MS separation of ribonuclease B-glycoforms of the same mass (positional or structural isomers).
AB - Successive multiple ionic-polymer layers (SMIL) coatings have been often used in capillary electrophoresis due to their simplicity to implement and regenerate. However, the performances of the separation are strongly dependent on the nature of the polyelectrolyte partners used to build the SMIL coating. In this work, we investigate new couples of polyelectrolytes that were not tested before: namely, polybrene (PB), quaternized diethylaminoethyl dextran (DEAEDq) and ε-poly(lysine) (ε-PLL), as polycations, in combination with poly(acrylic acid), dextran sulfate, poly(styrenesulfonate), poly(methacrylic acid) and poly(L-lysine citramide), as polyanions. Systematic study of intra- and inter-capillaries repeatabilities/reproducibilities was performed based on the determination of migration time, separation efficiency and electroosmotic mobility. Interestingly, the electroosmotic flow was found to vary with the nature of the polycation on a broad range of electroosmotic mobility decreasing in magnitude in the order of PB>ε-PLL > DEAEDq, whatever the polyanion associated. Application of the coatings to the separation of proteins is illustrated in a 0.5 M acetic acid BGE, including CE-MS separation of ribonuclease B-glycoforms of the same mass (positional or structural isomers).
KW - Capillary coatings
KW - Polyelectrolyte multilayers
KW - Polymer coatings
KW - Protein separation
UR - http://www.scopus.com/inward/record.url?scp=85060574653&partnerID=8YFLogxK
U2 - 10.1016/j.aca.2019.01.008
DO - 10.1016/j.aca.2019.01.008
M3 - Journal article
C2 - 30832914
AN - SCOPUS:85060574653
VL - 1057
SP - 152
EP - 161
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
ER -
ID: 389073131