Distinguishing between archaeological sheep and goat bones using a single collagen peptide
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Distinguishing between archaeological sheep and goat bones using a single collagen peptide. / Buckley, Mike; Whitcher Kansa, Sarah; Howard, Sarah; Campbell, Stuart; Thomas-Oates, Jane; Collins, Matthew.
In: Journal of Archaeological Science, Vol. 37, No. 1, 2010, p. 13-20.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Distinguishing between archaeological sheep and goat bones using a single collagen peptide
AU - Buckley, Mike
AU - Whitcher Kansa, Sarah
AU - Howard, Sarah
AU - Campbell, Stuart
AU - Thomas-Oates, Jane
AU - Collins, Matthew
PY - 2010
Y1 - 2010
N2 - We describe a method of isolating and analyzing a single collagen peptide able to distinguish between sheep and goat bone collagen. The 33 amino acid peptide from both sheep and goat collagen was sequenced and shown to differ between the two species at two positions. Analysis of a range of caprines indicated that the sequence changes occurred between the divergence of the Himalayan tahr (Hemitragus jemlahicus) and the ibex (Capra ibex) and that the proposed goat marker is diagnostic of all Capra species and breeds. The survival of these markers in archaeological bones was tested using a set of 26 ovicaprid specimens from Domuztepe, a Neolithic site in south central Turkey. These markers were used to test the osteological determination of 24 of the Domuztepe bones, and determine the species for two immature specimens. The collagen-peptide method has advantages over other non-morphological methods of sheep/goat distinction because of the long-term survival of collagen over other biomolecules such as ancient DNA. The results also highlighted the problems in relying upon one morphological criterion, in this case on the distal radius, to distinguish between sheep and goat bones.
AB - We describe a method of isolating and analyzing a single collagen peptide able to distinguish between sheep and goat bone collagen. The 33 amino acid peptide from both sheep and goat collagen was sequenced and shown to differ between the two species at two positions. Analysis of a range of caprines indicated that the sequence changes occurred between the divergence of the Himalayan tahr (Hemitragus jemlahicus) and the ibex (Capra ibex) and that the proposed goat marker is diagnostic of all Capra species and breeds. The survival of these markers in archaeological bones was tested using a set of 26 ovicaprid specimens from Domuztepe, a Neolithic site in south central Turkey. These markers were used to test the osteological determination of 24 of the Domuztepe bones, and determine the species for two immature specimens. The collagen-peptide method has advantages over other non-morphological methods of sheep/goat distinction because of the long-term survival of collagen over other biomolecules such as ancient DNA. The results also highlighted the problems in relying upon one morphological criterion, in this case on the distal radius, to distinguish between sheep and goat bones.
KW - Archaeological proteomics
KW - Biomolecular archaeology
KW - Collagen
KW - Domuztepe
KW - Goat
KW - MALDI
KW - Sheep
KW - Species identification
U2 - 10.1016/j.jas.2009.08.020
DO - 10.1016/j.jas.2009.08.020
M3 - Journal article
AN - SCOPUS:70350751437
VL - 37
SP - 13
EP - 20
JO - Journal of Archaeological Science
JF - Journal of Archaeological Science
SN - 0305-4403
IS - 1
ER -
ID: 229396855