Photosynthetic and transcriptional responses of the marine diatom Thalassiosira pseudonana to the combined effect of temperature stress and copper exposure
Research output: Contribution to journal › Journal article › Research › peer-review
A 96-h exposure experiment was conducted to elucidate the toxicity responses of the marine diatom Thalassiosira pseudonana upon exposure to different temperatures and copper (Cu) concentrations. Three Cu treatments (seawater control; 200μg/L Cu, EC50 for the yield at 25°C; and 1000μg/L Cu, EC50 for growth inhibition at 25°C) were conducted against four temperatures (10°C, 15°C, 25°C and 30°C). Growth rate and photosynthetic responses showed a significant interacting thermal-chemical effect with strong synergistic responses observed at 30°C treatments. Expression of heat shock protein (hsp) was positively modulated by increasing temperatures. Hsp 90, hsp90-2 and sit1 (related to silica shell formation) were highly expressed at 30°C under 1000μg/L Cu, while the genes encoding light harvesting proteins (3HfcpA and 3HfcpB) and silaffin precursor sil3 were significantly up-regulated at 15°C under 200μg/L Cu. Our results indicated an increase Cu toxicity to T. pseudonana under high temperature and Cu dose.
Original language | English |
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Journal | Marine Pollution Bulletin |
Volume | 124 |
Issue number | 2 |
Pages (from-to) | 938-945 |
Number of pages | 8 |
ISSN | 0025-326X |
DOIs | |
Publication status | Published - 30 Nov 2017 |
- Copper, Heat shock protein, Marine diatom, Photosynthesis, Thalassiosira, Thermal stress
Research areas
ID: 178742174