Increasing sustainability in palaeoproteomics by optimizing digestion times for large-scale archaeological bone analyses
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Increasing sustainability in palaeoproteomics by optimizing digestion times for large-scale archaeological bone analyses. / Le Meillour, Louise; Sinet-Mathiot, Virginie; Ásmundsdóttir, Ragnheiður Diljá; Hansen, Jakob; Mylopotamitaki, Dorothea; Troché, Gaudry; Xia, Huan; Herrera Bethencourt, Jorsua; Ruebens, Karen; Smith, Geoff M.; Fagernäs, Zandra; Welker, Frido.
In: iScience, Vol. 27, No. 4, 109432, 2024.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Increasing sustainability in palaeoproteomics by optimizing digestion times for large-scale archaeological bone analyses
AU - Le Meillour, Louise
AU - Sinet-Mathiot, Virginie
AU - Ásmundsdóttir, Ragnheiður Diljá
AU - Hansen, Jakob
AU - Mylopotamitaki, Dorothea
AU - Troché, Gaudry
AU - Xia, Huan
AU - Herrera Bethencourt, Jorsua
AU - Ruebens, Karen
AU - Smith, Geoff M.
AU - Fagernäs, Zandra
AU - Welker, Frido
N1 - Publisher Copyright: © 2024 The Author(s)
PY - 2024
Y1 - 2024
N2 - Palaeoproteomic analysis of skeletal proteomes is used to provide taxonomic identifications for an increasing number of archaeological specimens. The success rate depends on a range of taphonomic factors and differences in the extraction protocols employed. By analyzing 12 archaeological bone specimens from two archaeological sites, we demonstrate that reducing digestion duration from 18 to 3 hours has no measurable impact on the obtained taxonomic identifications. Peptide marker recovery, COL1 sequence coverage, or proteome complexity are also not significantly impacted. Although we observe minor differences in sequence coverage and glutamine deamidation, these are not consistent across our dataset. A 6-fold reduction in digestion time reduces electricity consumption, and therefore CO2 emission intensities. We furthermore demonstrate that working in 96-well plates further reduces electricity consumption by 60%, in comparison to individual microtubes. Reducing digestion time therefore has no impact on the taxonomic identifications, while reducing the environmental impact of palaeoproteomic projects.
AB - Palaeoproteomic analysis of skeletal proteomes is used to provide taxonomic identifications for an increasing number of archaeological specimens. The success rate depends on a range of taphonomic factors and differences in the extraction protocols employed. By analyzing 12 archaeological bone specimens from two archaeological sites, we demonstrate that reducing digestion duration from 18 to 3 hours has no measurable impact on the obtained taxonomic identifications. Peptide marker recovery, COL1 sequence coverage, or proteome complexity are also not significantly impacted. Although we observe minor differences in sequence coverage and glutamine deamidation, these are not consistent across our dataset. A 6-fold reduction in digestion time reduces electricity consumption, and therefore CO2 emission intensities. We furthermore demonstrate that working in 96-well plates further reduces electricity consumption by 60%, in comparison to individual microtubes. Reducing digestion time therefore has no impact on the taxonomic identifications, while reducing the environmental impact of palaeoproteomic projects.
KW - Archaeology
KW - Proteomics
U2 - 10.1016/j.isci.2024.109432
DO - 10.1016/j.isci.2024.109432
M3 - Journal article
AN - SCOPUS:85188552597
VL - 27
JO - iScience
JF - iScience
SN - 2589-0042
IS - 4
M1 - 109432
ER -
ID: 389591887