Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ1 Workstation

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Standard

Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ1 Workstation. / Stangegaard, Michael; Jørgensen, Mads; Hansen, Anders Johannes; Morling, Niels.

Progress in Forensic Genetics 13: Proceedings of the 23rd International ISFG Congress. ed. / Niels Morling. Elsevier, 2009. p. 69-70 (Forensic Science International: Genetics Supplement Series; No. 1, Vol. 2).

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Harvard

Stangegaard, M, Jørgensen, M, Hansen, AJ & Morling, N 2009, Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ1 Workstation. in N Morling (ed.), Progress in Forensic Genetics 13: Proceedings of the 23rd International ISFG Congress. Elsevier, Forensic Science International: Genetics Supplement Series, no. 1, vol. 2, pp. 69-70, 23rd International ISFG Congress, Buenos Aires, Argentina, 14/09/2009. https://doi.org/doi:10.1016/j.fsigss.2009.08.001

APA

Stangegaard, M., Jørgensen, M., Hansen, A. J., & Morling, N. (2009). Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ1 Workstation. In N. Morling (Ed.), Progress in Forensic Genetics 13: Proceedings of the 23rd International ISFG Congress (pp. 69-70). Elsevier. Forensic Science International: Genetics Supplement Series Vol. 2 No. 1 https://doi.org/doi:10.1016/j.fsigss.2009.08.001

Vancouver

Stangegaard M, Jørgensen M, Hansen AJ, Morling N. Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ1 Workstation. In Morling N, editor, Progress in Forensic Genetics 13: Proceedings of the 23rd International ISFG Congress. Elsevier. 2009. p. 69-70. (Forensic Science International: Genetics Supplement Series; No. 1, Vol. 2). https://doi.org/doi:10.1016/j.fsigss.2009.08.001

Author

Stangegaard, Michael ; Jørgensen, Mads ; Hansen, Anders Johannes ; Morling, Niels. / Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ1 Workstation. Progress in Forensic Genetics 13: Proceedings of the 23rd International ISFG Congress. editor / Niels Morling. Elsevier, 2009. pp. 69-70 (Forensic Science International: Genetics Supplement Series; No. 1, Vol. 2).

Bibtex

@inproceedings{cabfd160a1fd11debc73000ea68e967b,
title = "Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ1 Workstation",
abstract = "We have validated and implemented a protocol for DNA extraction from various types of biological materials using a Qiagen BioRobot EZ1 Workstation. The sample materials included whole blood, blood from deceased, buccal cells on Omni swabs and FTA Cards, blood on FTA Cards and cotton swabs, and muscle biopsies. The DNA extraction was validated according to EN/ISO 17025 for the STR kits AmpFlSTR« Identifiler« and AmpFlSTR« Yfiler« (Applied Biosystems). Of 298 samples extracted, 11 (4%) did not yield acceptable results. In conclusion, we have demonstrated that extraction of DNA from various types of biological material can be performed quickly and without the use of hazardous chemicals, and that the DNA may be successfully STR typed according to the requirements of forensic genetic investigations accredited according to EN/ISO 17025",
author = "Michael Stangegaard and Mads J{\o}rgensen and Hansen, {Anders Johannes} and Niels Morling",
year = "2009",
doi = "doi:10.1016/j.fsigss.2009.08.001",
language = "English",
series = "Forensic Science International: Genetics Supplement Series",
publisher = "Elsevier",
number = "1",
pages = "69--70",
editor = "Niels Morling",
booktitle = "Progress in Forensic Genetics 13",
address = "Netherlands",
note = "null ; Conference date: 14-09-2009 Through 18-09-2009",

}

RIS

TY - GEN

T1 - Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ1 Workstation

AU - Stangegaard, Michael

AU - Jørgensen, Mads

AU - Hansen, Anders Johannes

AU - Morling, Niels

PY - 2009

Y1 - 2009

N2 - We have validated and implemented a protocol for DNA extraction from various types of biological materials using a Qiagen BioRobot EZ1 Workstation. The sample materials included whole blood, blood from deceased, buccal cells on Omni swabs and FTA Cards, blood on FTA Cards and cotton swabs, and muscle biopsies. The DNA extraction was validated according to EN/ISO 17025 for the STR kits AmpFlSTR« Identifiler« and AmpFlSTR« Yfiler« (Applied Biosystems). Of 298 samples extracted, 11 (4%) did not yield acceptable results. In conclusion, we have demonstrated that extraction of DNA from various types of biological material can be performed quickly and without the use of hazardous chemicals, and that the DNA may be successfully STR typed according to the requirements of forensic genetic investigations accredited according to EN/ISO 17025

AB - We have validated and implemented a protocol for DNA extraction from various types of biological materials using a Qiagen BioRobot EZ1 Workstation. The sample materials included whole blood, blood from deceased, buccal cells on Omni swabs and FTA Cards, blood on FTA Cards and cotton swabs, and muscle biopsies. The DNA extraction was validated according to EN/ISO 17025 for the STR kits AmpFlSTR« Identifiler« and AmpFlSTR« Yfiler« (Applied Biosystems). Of 298 samples extracted, 11 (4%) did not yield acceptable results. In conclusion, we have demonstrated that extraction of DNA from various types of biological material can be performed quickly and without the use of hazardous chemicals, and that the DNA may be successfully STR typed according to the requirements of forensic genetic investigations accredited according to EN/ISO 17025

U2 - doi:10.1016/j.fsigss.2009.08.001

DO - doi:10.1016/j.fsigss.2009.08.001

M3 - Article in proceedings

T3 - Forensic Science International: Genetics Supplement Series

SP - 69

EP - 70

BT - Progress in Forensic Genetics 13

A2 - Morling, Niels

PB - Elsevier

Y2 - 14 September 2009 through 18 September 2009

ER -

ID: 14440302