Cyanidin based anthocyanin biosynthesis in orange carrot is restored by expression of AmRosea1 and AmDelila, MYB and bHLH transcription factors

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Cyanidin based anthocyanin biosynthesis in orange carrot is restored by expression of AmRosea1 and AmDelila, MYB and bHLH transcription factors. / Sharma, Shrikant; Holme, Inger B.; Dionisio, Giuseppe; Kodama, Miyako; Dzhanfezova, Tsaneta; Joernsgaard, Bjarne; Brinch-Pedersen, Henrik.

In: Plant Molecular Biology, Vol. 103, No. 4-5, 2020, p. 443-456.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Sharma, S, Holme, IB, Dionisio, G, Kodama, M, Dzhanfezova, T, Joernsgaard, B & Brinch-Pedersen, H 2020, 'Cyanidin based anthocyanin biosynthesis in orange carrot is restored by expression of AmRosea1 and AmDelila, MYB and bHLH transcription factors', Plant Molecular Biology, vol. 103, no. 4-5, pp. 443-456. https://doi.org/10.1007/s11103-020-01002-1

APA

Sharma, S., Holme, I. B., Dionisio, G., Kodama, M., Dzhanfezova, T., Joernsgaard, B., & Brinch-Pedersen, H. (2020). Cyanidin based anthocyanin biosynthesis in orange carrot is restored by expression of AmRosea1 and AmDelila, MYB and bHLH transcription factors. Plant Molecular Biology, 103(4-5), 443-456. https://doi.org/10.1007/s11103-020-01002-1

Vancouver

Sharma S, Holme IB, Dionisio G, Kodama M, Dzhanfezova T, Joernsgaard B et al. Cyanidin based anthocyanin biosynthesis in orange carrot is restored by expression of AmRosea1 and AmDelila, MYB and bHLH transcription factors. Plant Molecular Biology. 2020;103(4-5):443-456. https://doi.org/10.1007/s11103-020-01002-1

Author

Sharma, Shrikant ; Holme, Inger B. ; Dionisio, Giuseppe ; Kodama, Miyako ; Dzhanfezova, Tsaneta ; Joernsgaard, Bjarne ; Brinch-Pedersen, Henrik. / Cyanidin based anthocyanin biosynthesis in orange carrot is restored by expression of AmRosea1 and AmDelila, MYB and bHLH transcription factors. In: Plant Molecular Biology. 2020 ; Vol. 103, No. 4-5. pp. 443-456.

Bibtex

@article{cd29315c66234a6abc55a8f37de372e6,
title = "Cyanidin based anthocyanin biosynthesis in orange carrot is restored by expression of AmRosea1 and AmDelila, MYB and bHLH transcription factors",
abstract = "Key message The simultaneous expression ofAmRosea1andAmDelilatranscription factors from snapdragon can activate the anthocyanin pathway in orange carrots, leading to the synthesis and accumulation of anthocyanins in the taproots. Anthocyanins are phenolic compounds produced in various parts of plants. They are used as natural food dyes and are reported as beneficial antioxidants for humans. Black carrot is an important source for anthocyanins; however, the reason for the lack of anthocyanin production in the orange carrot is unknown. Anthocyanins are synthesized by a specific branch of the phenylpropanoid pathway that has previously been reported to be activated by a triad of R2R3-MYB, basic helix-loop helix (bHLH) and WD40 transcription factors (TFs). In the current study, orange carrots were turned purple by simultaneous expression of R2R3-MYB and bHLH TFs, i.e.AmRosea1andAmDelilafrom snapdragon (Antirrhinum majus). Simultaneous transgenic expression of the TFs under a constitutive promoter in the orange carrot cultivar 'Danvers 126' lead to consistent upregulation of anthocyanin-related biosynthetic genes and significant accumulation of anthocyanins in leaves, stems and taproots. Highest overall content of soluble anthocyanins in the taproot among the transformants amounted to 44.38 mg g(-1)dry weight. The anthocyanin profile of the transformants were significantly different from the profile in the reference black carrot 'Deep Purple'. The main anthocyanins present in the transformed taproots were cyanidin 3-xylosyl(sinapoylglucosyl)galactoside, whereas the main anthocyanin present in Deep Purple was cyanidin 3-xylosyl(feruloylglucosyl)galactoside. This study confirms the presence of the necessary biosynthetic genes in orange carrots for production of anthocyanins and demonstrates the absence of suitable R2R3-MYB and bHLH TFs for stimulating anthocyanin biosynthesis in the orange carrot.",
keywords = "AmRosea1, AmDelila, Anthocyanins, Daucus carotaL, Orange carrots, Black carrots, Transcription factors, DAUCUS-CAROTA, COLORFUL MODEL, GENES, IDENTIFICATION, PURPLE, ARABIDOPSIS, FLAVONOIDS, DECORATION, SELECTION, TOMATO",
author = "Shrikant Sharma and Holme, {Inger B.} and Giuseppe Dionisio and Miyako Kodama and Tsaneta Dzhanfezova and Bjarne Joernsgaard and Henrik Brinch-Pedersen",
year = "2020",
doi = "10.1007/s11103-020-01002-1",
language = "English",
volume = "103",
pages = "443--456",
journal = "Plant Molecular Biology",
issn = "0167-4412",
publisher = "Springer",
number = "4-5",

}

RIS

TY - JOUR

T1 - Cyanidin based anthocyanin biosynthesis in orange carrot is restored by expression of AmRosea1 and AmDelila, MYB and bHLH transcription factors

AU - Sharma, Shrikant

AU - Holme, Inger B.

AU - Dionisio, Giuseppe

AU - Kodama, Miyako

AU - Dzhanfezova, Tsaneta

AU - Joernsgaard, Bjarne

AU - Brinch-Pedersen, Henrik

PY - 2020

Y1 - 2020

N2 - Key message The simultaneous expression ofAmRosea1andAmDelilatranscription factors from snapdragon can activate the anthocyanin pathway in orange carrots, leading to the synthesis and accumulation of anthocyanins in the taproots. Anthocyanins are phenolic compounds produced in various parts of plants. They are used as natural food dyes and are reported as beneficial antioxidants for humans. Black carrot is an important source for anthocyanins; however, the reason for the lack of anthocyanin production in the orange carrot is unknown. Anthocyanins are synthesized by a specific branch of the phenylpropanoid pathway that has previously been reported to be activated by a triad of R2R3-MYB, basic helix-loop helix (bHLH) and WD40 transcription factors (TFs). In the current study, orange carrots were turned purple by simultaneous expression of R2R3-MYB and bHLH TFs, i.e.AmRosea1andAmDelilafrom snapdragon (Antirrhinum majus). Simultaneous transgenic expression of the TFs under a constitutive promoter in the orange carrot cultivar 'Danvers 126' lead to consistent upregulation of anthocyanin-related biosynthetic genes and significant accumulation of anthocyanins in leaves, stems and taproots. Highest overall content of soluble anthocyanins in the taproot among the transformants amounted to 44.38 mg g(-1)dry weight. The anthocyanin profile of the transformants were significantly different from the profile in the reference black carrot 'Deep Purple'. The main anthocyanins present in the transformed taproots were cyanidin 3-xylosyl(sinapoylglucosyl)galactoside, whereas the main anthocyanin present in Deep Purple was cyanidin 3-xylosyl(feruloylglucosyl)galactoside. This study confirms the presence of the necessary biosynthetic genes in orange carrots for production of anthocyanins and demonstrates the absence of suitable R2R3-MYB and bHLH TFs for stimulating anthocyanin biosynthesis in the orange carrot.

AB - Key message The simultaneous expression ofAmRosea1andAmDelilatranscription factors from snapdragon can activate the anthocyanin pathway in orange carrots, leading to the synthesis and accumulation of anthocyanins in the taproots. Anthocyanins are phenolic compounds produced in various parts of plants. They are used as natural food dyes and are reported as beneficial antioxidants for humans. Black carrot is an important source for anthocyanins; however, the reason for the lack of anthocyanin production in the orange carrot is unknown. Anthocyanins are synthesized by a specific branch of the phenylpropanoid pathway that has previously been reported to be activated by a triad of R2R3-MYB, basic helix-loop helix (bHLH) and WD40 transcription factors (TFs). In the current study, orange carrots were turned purple by simultaneous expression of R2R3-MYB and bHLH TFs, i.e.AmRosea1andAmDelilafrom snapdragon (Antirrhinum majus). Simultaneous transgenic expression of the TFs under a constitutive promoter in the orange carrot cultivar 'Danvers 126' lead to consistent upregulation of anthocyanin-related biosynthetic genes and significant accumulation of anthocyanins in leaves, stems and taproots. Highest overall content of soluble anthocyanins in the taproot among the transformants amounted to 44.38 mg g(-1)dry weight. The anthocyanin profile of the transformants were significantly different from the profile in the reference black carrot 'Deep Purple'. The main anthocyanins present in the transformed taproots were cyanidin 3-xylosyl(sinapoylglucosyl)galactoside, whereas the main anthocyanin present in Deep Purple was cyanidin 3-xylosyl(feruloylglucosyl)galactoside. This study confirms the presence of the necessary biosynthetic genes in orange carrots for production of anthocyanins and demonstrates the absence of suitable R2R3-MYB and bHLH TFs for stimulating anthocyanin biosynthesis in the orange carrot.

KW - AmRosea1

KW - AmDelila

KW - Anthocyanins

KW - Daucus carotaL

KW - Orange carrots

KW - Black carrots

KW - Transcription factors

KW - DAUCUS-CAROTA

KW - COLORFUL MODEL

KW - GENES

KW - IDENTIFICATION

KW - PURPLE

KW - ARABIDOPSIS

KW - FLAVONOIDS

KW - DECORATION

KW - SELECTION

KW - TOMATO

U2 - 10.1007/s11103-020-01002-1

DO - 10.1007/s11103-020-01002-1

M3 - Journal article

C2 - 32270430

VL - 103

SP - 443

EP - 456

JO - Plant Molecular Biology

JF - Plant Molecular Biology

SN - 0167-4412

IS - 4-5

ER -

ID: 247547075