DNA extraction from dry museum beetles without conferring external morphological damage.

Research output: Contribution to journal › Journal article › Research › peer-review

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DNA extraction from dry museum beetles without conferring external morphological damage. / Gilbert, M Thomas P; Moore, Wendy; Melchior, Linea; Worobey, Michael.

In: PLoS ONE, Vol. 2, No. 3, 2007, p. e272.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Gilbert, MTP, Moore, W, Melchior, L & Worobey, M 2007, 'DNA extraction from dry museum beetles without conferring external morphological damage.', PLoS ONE, vol. 2, no. 3, pp. e272. https://doi.org/10.1371/journal.pone.0000272

APA

Gilbert, M. T. P., Moore, W., Melchior, L., & Worobey, M. (2007). DNA extraction from dry museum beetles without conferring external morphological damage. PLoS ONE, 2(3), e272. https://doi.org/10.1371/journal.pone.0000272

Vancouver

Gilbert MTP, Moore W, Melchior L, Worobey M. DNA extraction from dry museum beetles without conferring external morphological damage. PLoS ONE. 2007;2(3):e272. https://doi.org/10.1371/journal.pone.0000272

Author

Gilbert, M Thomas P ; Moore, Wendy ; Melchior, Linea ; Worobey, Michael. / DNA extraction from dry museum beetles without conferring external morphological damage. In: PLoS ONE. 2007 ; Vol. 2, No. 3. pp. e272.

Bibtex

@article{e895a720149411ddbee902004c4f4f50,
title = "DNA extraction from dry museum beetles without conferring external morphological damage.",
abstract = "BACKGROUND: A large number of dry-preserved insect specimens exist in collections around the world that might be useful for genetic analyses. However, until now, the recovery of nucleic acids from such specimens has involved at least the partial destruction of the specimen. This is clearly undesirable when dealing with rare species or otherwise important specimens, such as type specimens. METHODOLOGY: We describe a method for the extraction of PCR-amplifiable mitochondrial and nuclear DNA from dry insects without causing external morphological damage. Using PCR to amplify approximately 220 bp of the mitochondrial gene cytochrome c oxidase I, and 250-345 bp fragments of the multi-copy, nuclear 28s ribosomal DNA gene, we demonstrate the efficacy of this method on beetles collected up to 50 years ago. CONCLUSIONS: This method offers a means of obtaining useful genetic information from rare insects without conferring external morphological damage. Udgivelsesdato: 2007-null",
author = "Gilbert, {M Thomas P} and Wendy Moore and Linea Melchior and Michael Worobey",
year = "2007",
doi = "10.1371/journal.pone.0000272",
language = "English",
volume = "2",
pages = "e272",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "3",

}

RIS

TY - JOUR

T1 - DNA extraction from dry museum beetles without conferring external morphological damage.

AU - Gilbert, M Thomas P

AU - Moore, Wendy

AU - Melchior, Linea

AU - Worobey, Michael

PY - 2007

Y1 - 2007

N2 - BACKGROUND: A large number of dry-preserved insect specimens exist in collections around the world that might be useful for genetic analyses. However, until now, the recovery of nucleic acids from such specimens has involved at least the partial destruction of the specimen. This is clearly undesirable when dealing with rare species or otherwise important specimens, such as type specimens. METHODOLOGY: We describe a method for the extraction of PCR-amplifiable mitochondrial and nuclear DNA from dry insects without causing external morphological damage. Using PCR to amplify approximately 220 bp of the mitochondrial gene cytochrome c oxidase I, and 250-345 bp fragments of the multi-copy, nuclear 28s ribosomal DNA gene, we demonstrate the efficacy of this method on beetles collected up to 50 years ago. CONCLUSIONS: This method offers a means of obtaining useful genetic information from rare insects without conferring external morphological damage. Udgivelsesdato: 2007-null

AB - BACKGROUND: A large number of dry-preserved insect specimens exist in collections around the world that might be useful for genetic analyses. However, until now, the recovery of nucleic acids from such specimens has involved at least the partial destruction of the specimen. This is clearly undesirable when dealing with rare species or otherwise important specimens, such as type specimens. METHODOLOGY: We describe a method for the extraction of PCR-amplifiable mitochondrial and nuclear DNA from dry insects without causing external morphological damage. Using PCR to amplify approximately 220 bp of the mitochondrial gene cytochrome c oxidase I, and 250-345 bp fragments of the multi-copy, nuclear 28s ribosomal DNA gene, we demonstrate the efficacy of this method on beetles collected up to 50 years ago. CONCLUSIONS: This method offers a means of obtaining useful genetic information from rare insects without conferring external morphological damage. Udgivelsesdato: 2007-null

U2 - 10.1371/journal.pone.0000272

DO - 10.1371/journal.pone.0000272

M3 - Journal article

C2 - 17342206

VL - 2

SP - e272

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 3

ER -

ID: 3848555