TY - JOUR

T1 - Dynamic Wolbachia prevalence in Acromyrmex leaf-cutting ants

T2 - potential for a nutritional symbiosis

AU - Andersen, Sandra Breum

AU - Boye, Mads

AU - Nash, David Richard

AU - Boomsma, Jacobus Jan

N1 - © 2012 The Authors. Journal of Evolutionary Biology © 2012 European Society For Evolutionary Biology.

PY - 2012

Y1 - 2012

N2 - Wolbachia are renowned as reproductive parasites, but their phenotypic effects in eusocial insects are not well understood. We used a combination of qrt-PCR, fluorescence in situ hybridization and laser scanning confocal microscopy to evaluate the dynamics of Wolbachia infections in the leaf-cutting ant Acromyrmex octospinosus across developmental stages of sterile workers. We confirm that workers are infected with one or two widespread wsp genotypes of Wolbachia, show that colony prevalence is always 100% and characterize two rare recombinant genotypes. One dominant genotype is always present and most abundant, whereas another only proliferates in adult workers of some colonies and is barely detectable in larvae and pupae. An explanation may be that Wolbachia genotypes compete for host resources in immature stages while adult tissues provide substantially more niche space. Tissue-specific prevalence of the two genotypes differs, with the rarer genotype being over-represented in the adult foregut and thorax muscles. Both genotypes occur extracellularly in the foregut, suggesting an unknown mutualistic function in worker ant nutrition. Both genotypes are also abundant in the faecal fluid of the ants, suggesting that they may have extended functional phenotypes in the fungus garden that the ants manure with their own faeces.

AB - Wolbachia are renowned as reproductive parasites, but their phenotypic effects in eusocial insects are not well understood. We used a combination of qrt-PCR, fluorescence in situ hybridization and laser scanning confocal microscopy to evaluate the dynamics of Wolbachia infections in the leaf-cutting ant Acromyrmex octospinosus across developmental stages of sterile workers. We confirm that workers are infected with one or two widespread wsp genotypes of Wolbachia, show that colony prevalence is always 100% and characterize two rare recombinant genotypes. One dominant genotype is always present and most abundant, whereas another only proliferates in adult workers of some colonies and is barely detectable in larvae and pupae. An explanation may be that Wolbachia genotypes compete for host resources in immature stages while adult tissues provide substantially more niche space. Tissue-specific prevalence of the two genotypes differs, with the rarer genotype being over-represented in the adult foregut and thorax muscles. Both genotypes occur extracellularly in the foregut, suggesting an unknown mutualistic function in worker ant nutrition. Both genotypes are also abundant in the faecal fluid of the ants, suggesting that they may have extended functional phenotypes in the fungus garden that the ants manure with their own faeces.

KW - Animals

KW - Ants

KW - In Situ Hybridization, Fluorescence

KW - Larva

KW - Pupa

KW - Symbiosis

KW - Wolbachia

U2 - 10.1111/j.1420-9101.2012.02521.x

DO - 10.1111/j.1420-9101.2012.02521.x

M3 - Journal article

C2 - 22530696

VL - 25

SP - 1340

EP - 1350

JO - Journal of Evolutionary Biology

JF - Journal of Evolutionary Biology

SN - 1010-061X

IS - 7

ER -