Our current understanding of the early peopling of the Americas includes sources, numbers, timings, and routes of the early migrations into the Americas. This knowledge is held primarily in light of recent genome-wide ancient DNA studies. Within the Americas, models of the diversification of founder population(s) and past population structure are informed by ancient DNA from the continents. Finally, in ongoing and future research initiatives, there is a need for active community engagement and partnerships to ensure inclusivity and long-term sustainability.