Enriching captivity conditions with natural elements does not prevent the loss of wild-like gut microbiota but shapes its compositional variation in two small mammals
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Enriching captivity conditions with natural elements does not prevent the loss of wild-like gut microbiota but shapes its compositional variation in two small mammals. / Koziol, Adam; Odriozola, Iñaki; Nyholm, Lasse; Leonard, Aoife; San Jose, Carlos; Pauperio, Joana; Ferreira, Clara; Hansen, Anders J.; Aizpurua, Ostaizka; Gilbert, M. Thomas P.; Alberdi, Antton.
In: MicrobiologyOpen, Vol. 11, No. 5, e1318, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Enriching captivity conditions with natural elements does not prevent the loss of wild-like gut microbiota but shapes its compositional variation in two small mammals
AU - Koziol, Adam
AU - Odriozola, Iñaki
AU - Nyholm, Lasse
AU - Leonard, Aoife
AU - San Jose, Carlos
AU - Pauperio, Joana
AU - Ferreira, Clara
AU - Hansen, Anders J.
AU - Aizpurua, Ostaizka
AU - Gilbert, M. Thomas P.
AU - Alberdi, Antton
PY - 2022
Y1 - 2022
N2 - As continued growth in gut microbiota studies in captive and model animals elucidates the importance of their role in host biology, further pursuit of how to retain a wild-like microbial community is becoming increasingly important to obtain representative results from captive animals. In this study, we assessed how the gut microbiota of two wild-caught small mammals, namely Crocidura russula (Eulipotyphla, insectivore) and Apodemus sylvaticus (Rodentia, omnivore), changed when bringing them into captivity. We analyzed fecal samples of 15 A. sylvaticus and 21 C. russula, immediately after bringing them into captivity and 5 weeks later, spread over two housing treatments: a "natural" setup enriched with elements freshly collected from nature and a "laboratory" setup with sterile artificial elements. Through sequencing of the V3-V4 region of the 16S recombinant RNA gene, we found that the initial microbial diversity dropped during captivity in both species, regardless of treatment. Community composition underwent a change of similar magnitude in both species and under both treatments. However, we did observe that the temporal development of the gut microbiome took different trajectories (i.e., changed in different directions) under different treatments, particularly in C. russula, suggesting that C. russula may be more susceptible to environmental change. The results of this experiment do not support the use of microbially enriched environments to retain wild-like microbial diversities and compositions, yet show that specific housing conditions can significantly affect the drift of microbial communities under captivity.
AB - As continued growth in gut microbiota studies in captive and model animals elucidates the importance of their role in host biology, further pursuit of how to retain a wild-like microbial community is becoming increasingly important to obtain representative results from captive animals. In this study, we assessed how the gut microbiota of two wild-caught small mammals, namely Crocidura russula (Eulipotyphla, insectivore) and Apodemus sylvaticus (Rodentia, omnivore), changed when bringing them into captivity. We analyzed fecal samples of 15 A. sylvaticus and 21 C. russula, immediately after bringing them into captivity and 5 weeks later, spread over two housing treatments: a "natural" setup enriched with elements freshly collected from nature and a "laboratory" setup with sterile artificial elements. Through sequencing of the V3-V4 region of the 16S recombinant RNA gene, we found that the initial microbial diversity dropped during captivity in both species, regardless of treatment. Community composition underwent a change of similar magnitude in both species and under both treatments. However, we did observe that the temporal development of the gut microbiome took different trajectories (i.e., changed in different directions) under different treatments, particularly in C. russula, suggesting that C. russula may be more susceptible to environmental change. The results of this experiment do not support the use of microbially enriched environments to retain wild-like microbial diversities and compositions, yet show that specific housing conditions can significantly affect the drift of microbial communities under captivity.
KW - 16S
KW - captivity
KW - diversity loss
KW - gut-microbiome
KW - host-associated microbiota
KW - non-model organism
KW - HOUSE-DUST
KW - HOST
KW - DIVERSITY
KW - COMMUNITY
KW - EXPOSURE
KW - INCREASES
KW - DEFENSE
KW - DISEASE
KW - SOIL
U2 - 10.1002/mbo3.1318
DO - 10.1002/mbo3.1318
M3 - Journal article
C2 - 36314753
VL - 11
JO - MicrobiologyOpen
JF - MicrobiologyOpen
SN - 2045-8827
IS - 5
M1 - e1318
ER -
ID: 322556703