Genes under positive selection in Escherichia coli
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Genes under positive selection in Escherichia coli. / Petersen, Lise; Bollback, Jonathan P; Dimmic, Matt; Hubisz, Melissa; Nielsen, Rasmus.
In: Genome Research, Vol. 17, No. 9, 2007, p. 1336-43.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Genes under positive selection in Escherichia coli
AU - Petersen, Lise
AU - Bollback, Jonathan P
AU - Dimmic, Matt
AU - Hubisz, Melissa
AU - Nielsen, Rasmus
N1 - Keywords: Amino Acid Sequence; Bacterial Proteins; Escherichia coli K12; Genes, Bacterial; Genome, Bacterial; Molecular Sequence Data; Recombination, Genetic; Selection (Genetics); Sequence Analysis, DNA; Sequence Homology, Amino Acid
PY - 2007
Y1 - 2007
N2 - We used a comparative genomics approach to identify genes that are under positive selection in six strains of Escherichia coli and Shigella flexneri, including five strains that are human pathogens. We find that positive selection targets a wide range of different functions in the E. coli genome, including cell surface proteins such as beta barrel porins, presumably because of the involvement of these genes in evolutionary arms races with other bacteria, phages, and/or the host immune system. Structural mapping of positively selected sites on trans-membrane beta barrel porins reveals that the residues under positive selection occur almost exclusively in the extracellular region of the proteins that are enriched with sites known to be targets of phages, colicins, or the host immune system. More surprisingly, we also find a number of other categories of genes that show very strong evidence for positive selection, such as the enigmatic rhs elements and transposases. Based on structural evidence, we hypothesize that the selection acting on transposases is related to the genomic conflict between transposable elements and the host genome.
AB - We used a comparative genomics approach to identify genes that are under positive selection in six strains of Escherichia coli and Shigella flexneri, including five strains that are human pathogens. We find that positive selection targets a wide range of different functions in the E. coli genome, including cell surface proteins such as beta barrel porins, presumably because of the involvement of these genes in evolutionary arms races with other bacteria, phages, and/or the host immune system. Structural mapping of positively selected sites on trans-membrane beta barrel porins reveals that the residues under positive selection occur almost exclusively in the extracellular region of the proteins that are enriched with sites known to be targets of phages, colicins, or the host immune system. More surprisingly, we also find a number of other categories of genes that show very strong evidence for positive selection, such as the enigmatic rhs elements and transposases. Based on structural evidence, we hypothesize that the selection acting on transposases is related to the genomic conflict between transposable elements and the host genome.
U2 - 10.1101/gr.6254707
DO - 10.1101/gr.6254707
M3 - Journal article
C2 - 17675366
VL - 17
SP - 1336
EP - 1343
JO - Genome Research
JF - Genome Research
SN - 1088-9051
IS - 9
ER -
ID: 11529270