Genome sequencing and comparison of two nonhuman primate animal models, the cynomolgus and Chinese rhesus macaques
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Genome sequencing and comparison of two nonhuman primate animal models, the cynomolgus and Chinese rhesus macaques. / Yan, Guangmei; Zhang, Guojie; Fang, Xiaodong; Zhang, Yanfeng; Li, Cai; Ling, Fei; Cooper, David N; Li, Qiye; Li, Yan; van Gool, Alain J.; Du, Hongli; Chen, Jiesi; Chen, Ronghua; Zhang, Pei; Huang, Zhiyong; Thompson, John R; Meng, Yuhuan; Bai, Yinqi; Wang, Jufang; Zhuo, Min; Wang, Tao; Huang, Ying; Wei, Liqiong; Li, Jianwen; Wang, Zhiwen; Hu, Haofu; Yang, Pengcheng; Le, Liang; Stenson, Peter D; Li, Bo; Liu, Xiaoming; Ball, Edward V; An, Na; Huang, Quanfei; Zhang, Yong; Fan, Wei; Zhang, Xiuqing; Li, Yingrui; Wang, Wen; Katze, Michael G; Su, Bing; Nielsen, Rasmus; Yang, Huanming; Wang, Jun; Wang, Xiaoning; Wang, Jian.
In: Nature Biotechnology, Vol. 29, No. 11, 2011, p. 1019-1023.Research output: Contribution to journal › Letter › Research › peer-review
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TY - JOUR
T1 - Genome sequencing and comparison of two nonhuman primate animal models, the cynomolgus and Chinese rhesus macaques
AU - Yan, Guangmei
AU - Zhang, Guojie
AU - Fang, Xiaodong
AU - Zhang, Yanfeng
AU - Li, Cai
AU - Ling, Fei
AU - Cooper, David N
AU - Li, Qiye
AU - Li, Yan
AU - van Gool, Alain J.
AU - Du, Hongli
AU - Chen, Jiesi
AU - Chen, Ronghua
AU - Zhang, Pei
AU - Huang, Zhiyong
AU - Thompson, John R
AU - Meng, Yuhuan
AU - Bai, Yinqi
AU - Wang, Jufang
AU - Zhuo, Min
AU - Wang, Tao
AU - Huang, Ying
AU - Wei, Liqiong
AU - Li, Jianwen
AU - Wang, Zhiwen
AU - Hu, Haofu
AU - Yang, Pengcheng
AU - Le, Liang
AU - Stenson, Peter D
AU - Li, Bo
AU - Liu, Xiaoming
AU - Ball, Edward V
AU - An, Na
AU - Huang, Quanfei
AU - Zhang, Yong
AU - Fan, Wei
AU - Zhang, Xiuqing
AU - Li, Yingrui
AU - Wang, Wen
AU - Katze, Michael G
AU - Su, Bing
AU - Nielsen, Rasmus
AU - Yang, Huanming
AU - Wang, Jun
AU - Wang, Xiaoning
AU - Wang, Jian
PY - 2011
Y1 - 2011
N2 - The nonhuman primates most commonly used in medical research are from the genus Macaca. To better understand the genetic differences between these animal models, we present high-quality draft genome sequences from two macaque species, the cynomolgus/crab-eating macaque and the Chinese rhesus macaque. Comparison with the previously sequenced Indian rhesus macaque reveals that all three macaques maintain abundant genetic heterogeneity, including millions of single-nucleotide substitutions and many insertions, deletions and gross chromosomal rearrangements. By assessing genetic regions with reduced variability, we identify genes in each macaque species that may have experienced positive selection. Genetic divergence patterns suggest that the cynomolgus macaque genome has been shaped by introgression after hybridization with the Chinese rhesus macaque. Macaque genes display a high degree of sequence similarity with human disease gene orthologs and drug targets. However, we identify several putatively dysfunctional genetic differences between the three macaque species, which may explain functional differences between them previously observed in clinical studies.
AB - The nonhuman primates most commonly used in medical research are from the genus Macaca. To better understand the genetic differences between these animal models, we present high-quality draft genome sequences from two macaque species, the cynomolgus/crab-eating macaque and the Chinese rhesus macaque. Comparison with the previously sequenced Indian rhesus macaque reveals that all three macaques maintain abundant genetic heterogeneity, including millions of single-nucleotide substitutions and many insertions, deletions and gross chromosomal rearrangements. By assessing genetic regions with reduced variability, we identify genes in each macaque species that may have experienced positive selection. Genetic divergence patterns suggest that the cynomolgus macaque genome has been shaped by introgression after hybridization with the Chinese rhesus macaque. Macaque genes display a high degree of sequence similarity with human disease gene orthologs and drug targets. However, we identify several putatively dysfunctional genetic differences between the three macaque species, which may explain functional differences between them previously observed in clinical studies.
KW - Animals
KW - Base Sequence
KW - Chromosome Aberrations
KW - Evolution, Molecular
KW - Genome
KW - Humans
KW - Macaca fascicularis
KW - Macaca mulatta
KW - Models, Animal
KW - Molecular Sequence Data
KW - Polymorphism, Genetic
KW - Sequence Analysis, DNA
KW - Species Specificity
U2 - 10.1038/nbt.1992
DO - 10.1038/nbt.1992
M3 - Letter
C2 - 22002653
VL - 29
SP - 1019
EP - 1023
JO - Nature Biotechnology
JF - Nature Biotechnology
SN - 1087-0156
IS - 11
ER -
ID: 43544318