High-throughput sequencing of core STR loci for forensic genetic investigations using the Roche Genome Sequencer FLX platform
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High-throughput sequencing of core STR loci for forensic genetic investigations using the Roche Genome Sequencer FLX platform. / Fordyce, Sarah Louise; Avila Arcos, Maria del Carmen; Rockenbauer, Eszter; Børsting, Claus; Frank-Hansen, Rune; Petersen, Johan Frederik Torp; Willerslev, Eske; Hansen, Anders Johannes; Morling, Niels; Gilbert, Tom.
In: BioTechniques, Vol. 51, No. 2, 01.08.2011, p. 127-133.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - High-throughput sequencing of core STR loci for forensic genetic investigations using the Roche Genome Sequencer FLX platform
AU - Fordyce, Sarah Louise
AU - Avila Arcos, Maria del Carmen
AU - Rockenbauer, Eszter
AU - Børsting, Claus
AU - Frank-Hansen, Rune
AU - Petersen, Johan Frederik Torp
AU - Willerslev, Eske
AU - Hansen, Anders Johannes
AU - Morling, Niels
AU - Gilbert, Tom
PY - 2011/8/1
Y1 - 2011/8/1
N2 - The analysis and profiling of short tandem repeat (STR) loci is routinely used in forensic genetics. Current methods to investigate STR loci, including PCR-based standard fragment analyses and capillary electrophoresis, only provide amplicon lengths that are used to estimate the number of STR repeat units. These methods do not allow for the full resolution of STR base composition that sequencing approaches could provide. Here we present an STR profiling method based on the use of the Roche Genome Sequencer (GS) FLX to simultaneously sequence multiple core STR loci. Using this method in combination with a bioinformatic tool designed specifically to analyze sequence lengths and frequencies, we found that GS FLX STR sequence data are comparable to conventional capillary electrophoresis-based STR typing. Furthermore, we found DNA base substitutions and repeat sequence variations that would not have been identified using conventional STR typing.
AB - The analysis and profiling of short tandem repeat (STR) loci is routinely used in forensic genetics. Current methods to investigate STR loci, including PCR-based standard fragment analyses and capillary electrophoresis, only provide amplicon lengths that are used to estimate the number of STR repeat units. These methods do not allow for the full resolution of STR base composition that sequencing approaches could provide. Here we present an STR profiling method based on the use of the Roche Genome Sequencer (GS) FLX to simultaneously sequence multiple core STR loci. Using this method in combination with a bioinformatic tool designed specifically to analyze sequence lengths and frequencies, we found that GS FLX STR sequence data are comparable to conventional capillary electrophoresis-based STR typing. Furthermore, we found DNA base substitutions and repeat sequence variations that would not have been identified using conventional STR typing.
U2 - 10.2144/000113721
DO - 10.2144/000113721
M3 - Journal article
C2 - 21806557
VL - 51
SP - 127
EP - 133
JO - BioTechniques
JF - BioTechniques
SN - 0736-6205
IS - 2
ER -
ID: 33882847