TY - JOUR

T1 - Increased Bacterial Richness Associated With Lesions Within the Porites spp. of Vietnam

AU - Rasmussen, Linett

AU - Barnes, Christopher

AU - Mak, Sarah Siu Tze

AU - Kjartansdóttir, Kristín Rós

AU - Hansen, Thomas Arn

AU - Doan-Nhu, Hai

AU - Nguyen-Ngoc, Lam

AU - Guldberg Frøslev, Tobias

AU - Hellström, Micaela

AU - Hansen, Anders Johannes

PY - 2020/5/27

Y1 - 2020/5/27

N2 - Coral reefs worldwide are rapidly declining due to increasing anthropogenic stressors and environmental changes, with large-scale mortalities of coral reefs observed in many locations across the globe. It has become clear that the microbiome of corals is important in understanding the causes of coral infections, although its exact role is yet to be fully understood. Here, we characterize the bacteria and fungi associated with the non-lesional and lesional (identified by discoloration and tissue loss) tissues of coral species from Vietnam. Metabarcoding of the bacterial 16S rRNA gene and the fungal ITS rRNA gene region were performed. We sampled across two Porites species with potentially multiple causes of stresses, yet the bacterial compositions of lesional regions were consistently different from non-lesional areas of the same coral. These differences were driven by a considerable and significant increase in OTU richness within the lesional region compared to the non-lesional region. While no single OTU was consistently associated with lesional tissue, indicator analysis revealed that nine OTUs were significantly more persistent in the lesional regions that could represent useful bioindicators of stress. Meanwhile, there were no indicator OTUs in the non-lesional region. Further investigations are needed to determine whether changing bacterial communities play a mechanistic role in inducing lesioning, or are opportunistically colonizing stressed corals.

AB - Coral reefs worldwide are rapidly declining due to increasing anthropogenic stressors and environmental changes, with large-scale mortalities of coral reefs observed in many locations across the globe. It has become clear that the microbiome of corals is important in understanding the causes of coral infections, although its exact role is yet to be fully understood. Here, we characterize the bacteria and fungi associated with the non-lesional and lesional (identified by discoloration and tissue loss) tissues of coral species from Vietnam. Metabarcoding of the bacterial 16S rRNA gene and the fungal ITS rRNA gene region were performed. We sampled across two Porites species with potentially multiple causes of stresses, yet the bacterial compositions of lesional regions were consistently different from non-lesional areas of the same coral. These differences were driven by a considerable and significant increase in OTU richness within the lesional region compared to the non-lesional region. While no single OTU was consistently associated with lesional tissue, indicator analysis revealed that nine OTUs were significantly more persistent in the lesional regions that could represent useful bioindicators of stress. Meanwhile, there were no indicator OTUs in the non-lesional region. Further investigations are needed to determine whether changing bacterial communities play a mechanistic role in inducing lesioning, or are opportunistically colonizing stressed corals.

KW - bacteria

KW - fungi

KW - metabarcoding

KW - microbiome

KW - molecular ecology

U2 - 10.3389/fevo.2020.00151

DO - 10.3389/fevo.2020.00151

M3 - Journal article

AN - SCOPUS:85086161067

VL - 8

JO - Frontiers in Ecology and Evolution

JF - Frontiers in Ecology and Evolution

SN - 2296-701X

M1 - 151

ER -