Isolation of nucleic acids and cultures from fossil ice and permafrost

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Isolation of nucleic acids and cultures from fossil ice and permafrost. / Willerslev, E.; Hansen, Anders J.; Poinar, H. N.

In: Trends in Ecology & Evolution, Vol. 19, No. 3, 2004, p. 2004 Mar;19(3):141-7.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Willerslev, E, Hansen, AJ & Poinar, HN 2004, 'Isolation of nucleic acids and cultures from fossil ice and permafrost', Trends in Ecology & Evolution, vol. 19, no. 3, pp. 2004 Mar;19(3):141-7. https://doi.org/10.1016/j.tree.2003.11.010

APA

Willerslev, E., Hansen, A. J., & Poinar, H. N. (2004). Isolation of nucleic acids and cultures from fossil ice and permafrost. Trends in Ecology & Evolution, 19(3), 2004 Mar;19(3):141-7. https://doi.org/10.1016/j.tree.2003.11.010

Vancouver

Willerslev E, Hansen AJ, Poinar HN. Isolation of nucleic acids and cultures from fossil ice and permafrost. Trends in Ecology & Evolution. 2004;19(3):2004 Mar;19(3):141-7. https://doi.org/10.1016/j.tree.2003.11.010

Author

Willerslev, E. ; Hansen, Anders J. ; Poinar, H. N. / Isolation of nucleic acids and cultures from fossil ice and permafrost. In: Trends in Ecology & Evolution. 2004 ; Vol. 19, No. 3. pp. 2004 Mar;19(3):141-7.

Bibtex

@article{4b18b2f098c611debc73000ea68e967b,
title = "Isolation of nucleic acids and cultures from fossil ice and permafrost",
abstract = "Owing to their constant low temperatures, glacial ice and permafrost might contain the oldest nucleic acids and microbial cells on Earth, which could prove key to reconstructing past ecosystems and for the planning of missions to other planets. However, recent claims concerning viable cells and microbial nucleic acids obtained from ice- and permafrost cores from hundreds of thousands to millions of years old are not properly authenticated and the findings could be the result of contamination. Here, we discuss the processes that restrict the long-term survival of DNA and/or RNA molecules in ice and permafrost, and highlight sources of contamination that could result in false claims. Additionally, we present a set of precautions, controls and criteria to help ensure that future cultures and sequences are authentic. Udgivelsesdato: 2004 Mar",
author = "E. Willerslev and Hansen, {Anders J.} and Poinar, {H. N.}",
note = "802SS Times Cited:42 Cited References Count:52",
year = "2004",
doi = "10.1016/j.tree.2003.11.010",
language = "English",
volume = "19",
pages = "2004 Mar;19(3):141--7",
journal = "Trends in Ecology & Evolution",
issn = "0169-5347",
publisher = "Elsevier Ltd. * Trends Journals",
number = "3",

}

RIS

TY - JOUR

T1 - Isolation of nucleic acids and cultures from fossil ice and permafrost

AU - Willerslev, E.

AU - Hansen, Anders J.

AU - Poinar, H. N.

N1 - 802SS Times Cited:42 Cited References Count:52

PY - 2004

Y1 - 2004

N2 - Owing to their constant low temperatures, glacial ice and permafrost might contain the oldest nucleic acids and microbial cells on Earth, which could prove key to reconstructing past ecosystems and for the planning of missions to other planets. However, recent claims concerning viable cells and microbial nucleic acids obtained from ice- and permafrost cores from hundreds of thousands to millions of years old are not properly authenticated and the findings could be the result of contamination. Here, we discuss the processes that restrict the long-term survival of DNA and/or RNA molecules in ice and permafrost, and highlight sources of contamination that could result in false claims. Additionally, we present a set of precautions, controls and criteria to help ensure that future cultures and sequences are authentic. Udgivelsesdato: 2004 Mar

AB - Owing to their constant low temperatures, glacial ice and permafrost might contain the oldest nucleic acids and microbial cells on Earth, which could prove key to reconstructing past ecosystems and for the planning of missions to other planets. However, recent claims concerning viable cells and microbial nucleic acids obtained from ice- and permafrost cores from hundreds of thousands to millions of years old are not properly authenticated and the findings could be the result of contamination. Here, we discuss the processes that restrict the long-term survival of DNA and/or RNA molecules in ice and permafrost, and highlight sources of contamination that could result in false claims. Additionally, we present a set of precautions, controls and criteria to help ensure that future cultures and sequences are authentic. Udgivelsesdato: 2004 Mar

U2 - 10.1016/j.tree.2003.11.010

DO - 10.1016/j.tree.2003.11.010

M3 - Journal article

C2 - 16701245

VL - 19

SP - 2004 Mar;19(3):141-7

JO - Trends in Ecology & Evolution

JF - Trends in Ecology & Evolution

SN - 0169-5347

IS - 3

ER -

ID: 14152594