Molecular diet analysis of two African free-tailed bats (Molossidae) using high throughput sequencing

Research output: Contribution to journalJournal articlepeer-review

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Molecular diet analysis of two African free-tailed bats (Molossidae) using high throughput sequencing. / Bohmann, Kristine; Monadjem, Ara; Noer, Christina Lehmkuhl; Rasmussen, Morten; Zeale, Matt R. K.; Clare, Elizabeth; Jones, Gareth; Willerslev, Eske; Gilbert, M. Thomas P.

In: PLoS ONE, Vol. 6, No. 6, 2011, p. e21441.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Bohmann, K, Monadjem, A, Noer, CL, Rasmussen, M, Zeale, MRK, Clare, E, Jones, G, Willerslev, E & Gilbert, MTP 2011, 'Molecular diet analysis of two African free-tailed bats (Molossidae) using high throughput sequencing', PLoS ONE, vol. 6, no. 6, pp. e21441. https://doi.org/10.1371/journal.pone.0021441

APA

Bohmann, K., Monadjem, A., Noer, C. L., Rasmussen, M., Zeale, M. R. K., Clare, E., Jones, G., Willerslev, E., & Gilbert, M. T. P. (2011). Molecular diet analysis of two African free-tailed bats (Molossidae) using high throughput sequencing. PLoS ONE, 6(6), e21441. https://doi.org/10.1371/journal.pone.0021441

Vancouver

Bohmann K, Monadjem A, Noer CL, Rasmussen M, Zeale MRK, Clare E et al. Molecular diet analysis of two African free-tailed bats (Molossidae) using high throughput sequencing. PLoS ONE. 2011;6(6):e21441. https://doi.org/10.1371/journal.pone.0021441

Author

Bohmann, Kristine ; Monadjem, Ara ; Noer, Christina Lehmkuhl ; Rasmussen, Morten ; Zeale, Matt R. K. ; Clare, Elizabeth ; Jones, Gareth ; Willerslev, Eske ; Gilbert, M. Thomas P. / Molecular diet analysis of two African free-tailed bats (Molossidae) using high throughput sequencing. In: PLoS ONE. 2011 ; Vol. 6, No. 6. pp. e21441.

Bibtex

@article{ae3df823d49744ca93eeef4bf2cc343a,
title = "Molecular diet analysis of two African free-tailed bats (Molossidae) using high throughput sequencing",
abstract = "Given the diversity of prey consumed by insectivorous bats, it is difficult to discern the composition of their diet using morphological or conventional PCR-based analyses of their faeces. We demonstrate the use of a powerful alternate tool, the use of the Roche FLX sequencing platform to deep-sequence uniquely 5′ tagged insect-generic barcode cytochrome c oxidase I (COI) fragments, that were PCR amplified from faecal pellets of two free-tailed bat species Chaerephon pumilus and Mops condylurus (family: Molossidae). Although the analyses were challenged by the paucity of southern African insect COI sequences in the GenBank and BOLD databases, similarity to existing collections allowed the preliminary identification of 25 prey families from six orders of insects within the diet of C. pumilus, and 24 families from seven orders within the diet of M. condylurus. Insects identified to families within the orders Lepidoptera and Diptera were widely present among the faecal samples analysed. The two families that were observed most frequently were Noctuidae and Nymphalidae (Lepidoptera). Species-level analysis of the data was accomplished using novel bioinformatics techniques for the identification of molecular operational taxonomic units (MOTU). Based on these analyses, our data provide little evidence of resource partitioning between sympatric M. condylurus and C. pumilus in the Simunye region of Swaziland at the time of year when the samples were collected, although as more complete databases against which to compare the sequences are generated this may have to be re-evaluated. {\textcopyright} 2011 Bohmann et al.",
author = "Kristine Bohmann and Ara Monadjem and Noer, {Christina Lehmkuhl} and Morten Rasmussen and Zeale, {Matt R. K.} and Elizabeth Clare and Gareth Jones and Eske Willerslev and Gilbert, {M. Thomas P.}",
year = "2011",
doi = "10.1371/journal.pone.0021441",
language = "English",
volume = "6",
pages = "e21441",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "6",

}

RIS

TY - JOUR

T1 - Molecular diet analysis of two African free-tailed bats (Molossidae) using high throughput sequencing

AU - Bohmann, Kristine

AU - Monadjem, Ara

AU - Noer, Christina Lehmkuhl

AU - Rasmussen, Morten

AU - Zeale, Matt R. K.

AU - Clare, Elizabeth

AU - Jones, Gareth

AU - Willerslev, Eske

AU - Gilbert, M. Thomas P.

PY - 2011

Y1 - 2011

N2 - Given the diversity of prey consumed by insectivorous bats, it is difficult to discern the composition of their diet using morphological or conventional PCR-based analyses of their faeces. We demonstrate the use of a powerful alternate tool, the use of the Roche FLX sequencing platform to deep-sequence uniquely 5′ tagged insect-generic barcode cytochrome c oxidase I (COI) fragments, that were PCR amplified from faecal pellets of two free-tailed bat species Chaerephon pumilus and Mops condylurus (family: Molossidae). Although the analyses were challenged by the paucity of southern African insect COI sequences in the GenBank and BOLD databases, similarity to existing collections allowed the preliminary identification of 25 prey families from six orders of insects within the diet of C. pumilus, and 24 families from seven orders within the diet of M. condylurus. Insects identified to families within the orders Lepidoptera and Diptera were widely present among the faecal samples analysed. The two families that were observed most frequently were Noctuidae and Nymphalidae (Lepidoptera). Species-level analysis of the data was accomplished using novel bioinformatics techniques for the identification of molecular operational taxonomic units (MOTU). Based on these analyses, our data provide little evidence of resource partitioning between sympatric M. condylurus and C. pumilus in the Simunye region of Swaziland at the time of year when the samples were collected, although as more complete databases against which to compare the sequences are generated this may have to be re-evaluated. © 2011 Bohmann et al.

AB - Given the diversity of prey consumed by insectivorous bats, it is difficult to discern the composition of their diet using morphological or conventional PCR-based analyses of their faeces. We demonstrate the use of a powerful alternate tool, the use of the Roche FLX sequencing platform to deep-sequence uniquely 5′ tagged insect-generic barcode cytochrome c oxidase I (COI) fragments, that were PCR amplified from faecal pellets of two free-tailed bat species Chaerephon pumilus and Mops condylurus (family: Molossidae). Although the analyses were challenged by the paucity of southern African insect COI sequences in the GenBank and BOLD databases, similarity to existing collections allowed the preliminary identification of 25 prey families from six orders of insects within the diet of C. pumilus, and 24 families from seven orders within the diet of M. condylurus. Insects identified to families within the orders Lepidoptera and Diptera were widely present among the faecal samples analysed. The two families that were observed most frequently were Noctuidae and Nymphalidae (Lepidoptera). Species-level analysis of the data was accomplished using novel bioinformatics techniques for the identification of molecular operational taxonomic units (MOTU). Based on these analyses, our data provide little evidence of resource partitioning between sympatric M. condylurus and C. pumilus in the Simunye region of Swaziland at the time of year when the samples were collected, although as more complete databases against which to compare the sequences are generated this may have to be re-evaluated. © 2011 Bohmann et al.

U2 - 10.1371/journal.pone.0021441

DO - 10.1371/journal.pone.0021441

M3 - Journal article

C2 - 21731749

VL - 6

SP - e21441

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 6

ER -

ID: 33911683