Multi-omic detection of Mycobacterium leprae in archaeological human dental calculus

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Multi-omic detection of Mycobacterium leprae in archaeological human dental calculus. / Fotakis, Anna K.; Denham, Sean D.; Mackie, Meaghan; Orbegozo, Miren Iraeta; Mylopotamitaki, Dorothea; Gopalakrishnan, Shyam; Sicheritz-Pontén, Thomas; Olsen, Jesper V.; Cappellini, Enrico; Zhang, Guojie; Christophersen, Axel; Gilbert, M. Thomas P.; Vågene, Åshild J.

In: Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 375, No. 1812, 20190584, 2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Fotakis, AK, Denham, SD, Mackie, M, Orbegozo, MI, Mylopotamitaki, D, Gopalakrishnan, S, Sicheritz-Pontén, T, Olsen, JV, Cappellini, E, Zhang, G, Christophersen, A, Gilbert, MTP & Vågene, ÅJ 2020, 'Multi-omic detection of Mycobacterium leprae in archaeological human dental calculus', Philosophical Transactions of the Royal Society B: Biological Sciences, vol. 375, no. 1812, 20190584. https://doi.org/10.1098/rstb.2019.0584

APA

Fotakis, A. K., Denham, S. D., Mackie, M., Orbegozo, M. I., Mylopotamitaki, D., Gopalakrishnan, S., Sicheritz-Pontén, T., Olsen, J. V., Cappellini, E., Zhang, G., Christophersen, A., Gilbert, M. T. P., & Vågene, Å. J. (2020). Multi-omic detection of Mycobacterium leprae in archaeological human dental calculus. Philosophical Transactions of the Royal Society B: Biological Sciences, 375(1812), [20190584]. https://doi.org/10.1098/rstb.2019.0584

Vancouver

Fotakis AK, Denham SD, Mackie M, Orbegozo MI, Mylopotamitaki D, Gopalakrishnan S et al. Multi-omic detection of Mycobacterium leprae in archaeological human dental calculus. Philosophical Transactions of the Royal Society B: Biological Sciences. 2020;375(1812). 20190584. https://doi.org/10.1098/rstb.2019.0584

Author

Fotakis, Anna K. ; Denham, Sean D. ; Mackie, Meaghan ; Orbegozo, Miren Iraeta ; Mylopotamitaki, Dorothea ; Gopalakrishnan, Shyam ; Sicheritz-Pontén, Thomas ; Olsen, Jesper V. ; Cappellini, Enrico ; Zhang, Guojie ; Christophersen, Axel ; Gilbert, M. Thomas P. ; Vågene, Åshild J. / Multi-omic detection of Mycobacterium leprae in archaeological human dental calculus. In: Philosophical Transactions of the Royal Society B: Biological Sciences. 2020 ; Vol. 375, No. 1812.

Bibtex

@article{d66c8ed2d090469e9300009996b1a971,
title = "Multi-omic detection of Mycobacterium leprae in archaeological human dental calculus",
abstract = "Mineralized dental plaque (calculus) has proven to be an excellent source of ancient biomolecules. Here we present a Mycobacterium leprae genome (6.6-fold), the causative agent of leprosy, recovered via shotgun sequencing of sixteenth-century human dental calculus from an individual from Trondheim, Norway. When phylogenetically placed, this genome falls in branch 3I among the diversity of other contemporary ancient strains from Northern Europe. Moreover, ancient mycobacterial peptides were retrieved via mass spectrometry-based proteomics, further validating the presence of the pathogen. Mycobacterium leprae can readily be detected in the oral cavity and associated mucosal membranes, which likely contributed to it being incorporated into this individual's dental calculus. This individual showed some possible, but not definitive, evidence of skeletal lesions associated with early-stage leprosy. This study is the first known example of successful multi-omics retrieval of M. leprae from archaeological dental calculus. Furthermore, we offer new insights into dental calculus as an alternative sample source to bones or teeth for detecting and molecularly characterizing M. leprae in individuals from the archaeological record. This article is part of the theme issue 'Insights into health and disease from ancient biomolecules'.",
author = "Fotakis, {Anna K.} and Denham, {Sean D.} and Meaghan Mackie and Orbegozo, {Miren Iraeta} and Dorothea Mylopotamitaki and Shyam Gopalakrishnan and Thomas Sicheritz-Pont{\'e}n and Olsen, {Jesper V.} and Enrico Cappellini and Guojie Zhang and Axel Christophersen and Gilbert, {M. Thomas P.} and V{\aa}gene, {{\AA}shild J.}",
year = "2020",
doi = "10.1098/rstb.2019.0584",
language = "English",
volume = "375",
journal = "Philosophical Transactions of the Royal Society B: Biological Sciences",
issn = "0962-8436",
publisher = "The/Royal Society",
number = "1812",

}

RIS

TY - JOUR

T1 - Multi-omic detection of Mycobacterium leprae in archaeological human dental calculus

AU - Fotakis, Anna K.

AU - Denham, Sean D.

AU - Mackie, Meaghan

AU - Orbegozo, Miren Iraeta

AU - Mylopotamitaki, Dorothea

AU - Gopalakrishnan, Shyam

AU - Sicheritz-Pontén, Thomas

AU - Olsen, Jesper V.

AU - Cappellini, Enrico

AU - Zhang, Guojie

AU - Christophersen, Axel

AU - Gilbert, M. Thomas P.

AU - Vågene, Åshild J.

PY - 2020

Y1 - 2020

N2 - Mineralized dental plaque (calculus) has proven to be an excellent source of ancient biomolecules. Here we present a Mycobacterium leprae genome (6.6-fold), the causative agent of leprosy, recovered via shotgun sequencing of sixteenth-century human dental calculus from an individual from Trondheim, Norway. When phylogenetically placed, this genome falls in branch 3I among the diversity of other contemporary ancient strains from Northern Europe. Moreover, ancient mycobacterial peptides were retrieved via mass spectrometry-based proteomics, further validating the presence of the pathogen. Mycobacterium leprae can readily be detected in the oral cavity and associated mucosal membranes, which likely contributed to it being incorporated into this individual's dental calculus. This individual showed some possible, but not definitive, evidence of skeletal lesions associated with early-stage leprosy. This study is the first known example of successful multi-omics retrieval of M. leprae from archaeological dental calculus. Furthermore, we offer new insights into dental calculus as an alternative sample source to bones or teeth for detecting and molecularly characterizing M. leprae in individuals from the archaeological record. This article is part of the theme issue 'Insights into health and disease from ancient biomolecules'.

AB - Mineralized dental plaque (calculus) has proven to be an excellent source of ancient biomolecules. Here we present a Mycobacterium leprae genome (6.6-fold), the causative agent of leprosy, recovered via shotgun sequencing of sixteenth-century human dental calculus from an individual from Trondheim, Norway. When phylogenetically placed, this genome falls in branch 3I among the diversity of other contemporary ancient strains from Northern Europe. Moreover, ancient mycobacterial peptides were retrieved via mass spectrometry-based proteomics, further validating the presence of the pathogen. Mycobacterium leprae can readily be detected in the oral cavity and associated mucosal membranes, which likely contributed to it being incorporated into this individual's dental calculus. This individual showed some possible, but not definitive, evidence of skeletal lesions associated with early-stage leprosy. This study is the first known example of successful multi-omics retrieval of M. leprae from archaeological dental calculus. Furthermore, we offer new insights into dental calculus as an alternative sample source to bones or teeth for detecting and molecularly characterizing M. leprae in individuals from the archaeological record. This article is part of the theme issue 'Insights into health and disease from ancient biomolecules'.

U2 - 10.1098/rstb.2019.0584

DO - 10.1098/rstb.2019.0584

M3 - Journal article

C2 - 33012227

VL - 375

JO - Philosophical Transactions of the Royal Society B: Biological Sciences

JF - Philosophical Transactions of the Royal Society B: Biological Sciences

SN - 0962-8436

IS - 1812

M1 - 20190584

ER -

ID: 249481252