Non-invasive surveys of mammalian viruses using environmental DNA

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Non-invasive surveys of mammalian viruses using environmental DNA. / Alfano, Niccolò; Dayaram, Anisha; Axtner, Jan; Tsangaras, Kyriakos; Kampmann, Marie-Louise; Mohamed, Azlan; Wong, Seth T.; Gilbert, M. Thomas P.; Wilting, Andreas; Greenwood, Alex D.

In: Methods in Ecology and Evolution, Vol. 12, No. 10, 2021, p. 1941-1952.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Alfano, N, Dayaram, A, Axtner, J, Tsangaras, K, Kampmann, M-L, Mohamed, A, Wong, ST, Gilbert, MTP, Wilting, A & Greenwood, AD 2021, 'Non-invasive surveys of mammalian viruses using environmental DNA', Methods in Ecology and Evolution, vol. 12, no. 10, pp. 1941-1952. https://doi.org/10.1111/2041-210X.13661

APA

Alfano, N., Dayaram, A., Axtner, J., Tsangaras, K., Kampmann, M-L., Mohamed, A., Wong, S. T., Gilbert, M. T. P., Wilting, A., & Greenwood, A. D. (2021). Non-invasive surveys of mammalian viruses using environmental DNA. Methods in Ecology and Evolution, 12(10), 1941-1952. https://doi.org/10.1111/2041-210X.13661

Vancouver

Alfano N, Dayaram A, Axtner J, Tsangaras K, Kampmann M-L, Mohamed A et al. Non-invasive surveys of mammalian viruses using environmental DNA. Methods in Ecology and Evolution. 2021;12(10):1941-1952. https://doi.org/10.1111/2041-210X.13661

Author

Alfano, Niccolò ; Dayaram, Anisha ; Axtner, Jan ; Tsangaras, Kyriakos ; Kampmann, Marie-Louise ; Mohamed, Azlan ; Wong, Seth T. ; Gilbert, M. Thomas P. ; Wilting, Andreas ; Greenwood, Alex D. / Non-invasive surveys of mammalian viruses using environmental DNA. In: Methods in Ecology and Evolution. 2021 ; Vol. 12, No. 10. pp. 1941-1952.

Bibtex

@article{6d67677855ed414abf010d9f24d59f14,

title = "Non-invasive surveys of mammalian viruses using environmental DNA",

abstract = "Environmental DNA (eDNA) and invertebrate-derived DNA (iDNA) are used to survey biodiversity non-invasively to mitigate difficulties in obtaining wildlife samples, particularly in remote areas or for rare species. Recently, eDNA/iDNA were used to monitor known wildlife pathogens; however, most wildlife pathogens are unknown and often evolutionarily divergent. To detect and identify known and novel mammalian viruses from eDNA/iDNA, we used a curated set of RNA oligonucleotides as viral baits in a hybridization capture system coupled with high-throughput sequencing. We detected multiple known and novel mammalian RNA and DNA viruses from multiple viral families from both waterhole eDNA and leech-derived iDNA. Congruence was found between detected hosts and viruses identified in leeches and waterholes. Our results demonstrate that eDNA/iDNA samples represent an effective non-invasive resource for studying wildlife viral diversity and for detecting novel potentially zoonotic viruses prior to their emergence.",

keywords = "environmental DNA (eDNA), hybridization capture, leeches, non-invasive samples, viral diversity",

author = "Niccol{\`o} Alfano and Anisha Dayaram and Jan Axtner and Kyriakos Tsangaras and Marie-Louise Kampmann and Azlan Mohamed and Wong, {Seth T.} and Gilbert, {M. Thomas P.} and Andreas Wilting and Greenwood, {Alex D.}",

note = "Publisher Copyright: {\textcopyright} 2021 The Authors. Methods in Ecology and Evolution published by John Wiley & Sons Ltd on behalf of British Ecological Society",

year = "2021",

doi = "10.1111/2041-210X.13661",

language = "English",

volume = "12",

pages = "1941--1952",

journal = "Methods in Ecology and Evolution",

issn = "2041-210X",

publisher = "Wiley-Blackwell",

number = "10",

}

RIS

TY - JOUR

T1 - Non-invasive surveys of mammalian viruses using environmental DNA

AU - Alfano, Niccolò

AU - Dayaram, Anisha

AU - Axtner, Jan

AU - Tsangaras, Kyriakos

AU - Kampmann, Marie-Louise

AU - Mohamed, Azlan

AU - Wong, Seth T.

AU - Gilbert, M. Thomas P.

AU - Wilting, Andreas

AU - Greenwood, Alex D.

PY - 2021

Y1 - 2021

N2 - Environmental DNA (eDNA) and invertebrate-derived DNA (iDNA) are used to survey biodiversity non-invasively to mitigate difficulties in obtaining wildlife samples, particularly in remote areas or for rare species. Recently, eDNA/iDNA were used to monitor known wildlife pathogens; however, most wildlife pathogens are unknown and often evolutionarily divergent. To detect and identify known and novel mammalian viruses from eDNA/iDNA, we used a curated set of RNA oligonucleotides as viral baits in a hybridization capture system coupled with high-throughput sequencing. We detected multiple known and novel mammalian RNA and DNA viruses from multiple viral families from both waterhole eDNA and leech-derived iDNA. Congruence was found between detected hosts and viruses identified in leeches and waterholes. Our results demonstrate that eDNA/iDNA samples represent an effective non-invasive resource for studying wildlife viral diversity and for detecting novel potentially zoonotic viruses prior to their emergence.

AB - Environmental DNA (eDNA) and invertebrate-derived DNA (iDNA) are used to survey biodiversity non-invasively to mitigate difficulties in obtaining wildlife samples, particularly in remote areas or for rare species. Recently, eDNA/iDNA were used to monitor known wildlife pathogens; however, most wildlife pathogens are unknown and often evolutionarily divergent. To detect and identify known and novel mammalian viruses from eDNA/iDNA, we used a curated set of RNA oligonucleotides as viral baits in a hybridization capture system coupled with high-throughput sequencing. We detected multiple known and novel mammalian RNA and DNA viruses from multiple viral families from both waterhole eDNA and leech-derived iDNA. Congruence was found between detected hosts and viruses identified in leeches and waterholes. Our results demonstrate that eDNA/iDNA samples represent an effective non-invasive resource for studying wildlife viral diversity and for detecting novel potentially zoonotic viruses prior to their emergence.

KW - environmental DNA (eDNA)

KW - hybridization capture

KW - leeches

KW - non-invasive samples

KW - viral diversity

U2 - 10.1111/2041-210X.13661

DO - 10.1111/2041-210X.13661

M3 - Journal article

AN - SCOPUS:85111098179

VL - 12

SP - 1941

EP - 1952

JO - Methods in Ecology and Evolution

JF - Methods in Ecology and Evolution

SN - 2041-210X

IS - 10

ER -

ID: 276268197

Globe Institute