Phylogenetic origin and diversification of RNAi pathway genes in insects
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Phylogenetic origin and diversification of RNAi pathway genes in insects. / Dowling, Daniel; Pauli, Thomas; Donath, Alexander; Meusemann, Karen; Podsiadlowski, Lars; Petersen, Malte; Peters, Ralph S.; Mayer, Christoph; Liu, Shanlin; Zhou, Xin; Misof, Bernhard; Niehuis, Oliver.
In: Genome Biology and Evolution, Vol. 8, No. 12, 2016, p. 3784-3793.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Phylogenetic origin and diversification of RNAi pathway genes in insects
AU - Dowling, Daniel
AU - Pauli, Thomas
AU - Donath, Alexander
AU - Meusemann, Karen
AU - Podsiadlowski, Lars
AU - Petersen, Malte
AU - Peters, Ralph S.
AU - Mayer, Christoph
AU - Liu, Shanlin
AU - Zhou, Xin
AU - Misof, Bernhard
AU - Niehuis, Oliver
PY - 2016
Y1 - 2016
N2 - RNAinterference (RNAi) refers tothe set ofmolecular processes foundin eukaryotic organisms in which smallRNAmolecules mediate the silencing or down-regulation of target genes. In insects, RNAi serves a number of functions, including regulation of endogenous genes, anti-viral defense, and defense against transposable elements. Despite being well studied in model organisms, such as Drosophila, the distribution of core RNAi pathway genes and their evolution in insects is not well understood. Here we present the most comprehensive overview of the distribution and diversity of core RNAi pathway genes across 100 insect species, encompassing all currently recognized insect orders. We inferred the phylogenetic origin of insect-specific RNAi pathway genes and also identified several hitherto unrecorded gene expansions using whole-body transcriptome data from the international 1KITE (1000 Insect Transcriptome Evolution) project aswell asother resources such as i5K(5000InsectGenomeProject). Specifically,we traced the origin of the double stranded RNAbinding protein R2D2 to the lastcommon ancestor of winged insects (Pterygota), the loss of Sid-1/ Tag-130 orthologs in Antliophora (fleas, flies and relatives, and scorpionflies in a broad sense), and confirm previous evidence for the splitting of the Argonaute proteins Aubergine and Piwi in Brachyceran flies (Diptera, Brachycera). Our study offers new reference points for future experimental research on RNAi-related pathway genes in insects.
AB - RNAinterference (RNAi) refers tothe set ofmolecular processes foundin eukaryotic organisms in which smallRNAmolecules mediate the silencing or down-regulation of target genes. In insects, RNAi serves a number of functions, including regulation of endogenous genes, anti-viral defense, and defense against transposable elements. Despite being well studied in model organisms, such as Drosophila, the distribution of core RNAi pathway genes and their evolution in insects is not well understood. Here we present the most comprehensive overview of the distribution and diversity of core RNAi pathway genes across 100 insect species, encompassing all currently recognized insect orders. We inferred the phylogenetic origin of insect-specific RNAi pathway genes and also identified several hitherto unrecorded gene expansions using whole-body transcriptome data from the international 1KITE (1000 Insect Transcriptome Evolution) project aswell asother resources such as i5K(5000InsectGenomeProject). Specifically,we traced the origin of the double stranded RNAbinding protein R2D2 to the lastcommon ancestor of winged insects (Pterygota), the loss of Sid-1/ Tag-130 orthologs in Antliophora (fleas, flies and relatives, and scorpionflies in a broad sense), and confirm previous evidence for the splitting of the Argonaute proteins Aubergine and Piwi in Brachyceran flies (Diptera, Brachycera). Our study offers new reference points for future experimental research on RNAi-related pathway genes in insects.
KW - Argonaute
KW - Dicer
KW - Evolution
KW - R2d2
KW - RNA interference
U2 - 10.1093/gbe/evw281
DO - 10.1093/gbe/evw281
M3 - Journal article
C2 - 28062756
AN - SCOPUS:85014361427
VL - 8
SP - 3784
EP - 3793
JO - Genome Biology and Evolution
JF - Genome Biology and Evolution
SN - 1759-6653
IS - 12
ER -
ID: 178887793