TY - JOUR

T1 - Plastic nanoparticles cause mild inflammation, disrupt metabolic pathways, change the gut microbiota and affect reproduction in zebrafish

T2 - A full generation multi-omics study

AU - Marana, Moonika Haahr

AU - Poulsen, Rikke

AU - Thormar, Eiríkur Andri

AU - Clausen, Cecilie Grønlund

AU - Thit, Amalie

AU - Mathiessen, Heidi

AU - Jaafar, Rzgar

AU - Korbut, Rozalia

AU - Hansen, Anna Magdalene Brun

AU - Hansen, Martin

AU - Limborg, Morten Tønsberg

AU - Syberg, Kristian

AU - von Gersdorff Jørgensen, Louise

N1 - Publisher Copyright: © 2021 Elsevier B.V.

PY - 2022

Y1 - 2022

N2 - Plastic pollution has become a major concern on a global scale. The plastic is broken down into minuscule particles, which have an impact on the biosystems, however long-term impacts through an entire generation is largely unknown. Here, we present the first whole generation study exposing fish to a 500 nm polystyrene plastic particle at environmentally relevant concentrations. Short- and long-term adverse effects were investigated in the zebrafish model organism using a holistic multi-omics approach. The particles accumulated in the yolk sac of young larvae and short-term biological impacts included immune-relevant gene regulation related to inflammation and tolerance as well as disruption of metabolic processes, such as the fatty acid and lipid pathways. The long-term effects comprised gene regulations pointing towards skin and/or gill inflammation, dysbiosis of the gut microbiota, a tendency towards decreased condition factor in adult males as well as a lowered reproductive capability. From this study, it can be concluded that exposures to plastic nanoparticles have an impact on population as well as ecosystem level in fish and likely also in other vertebrates.

AB - Plastic pollution has become a major concern on a global scale. The plastic is broken down into minuscule particles, which have an impact on the biosystems, however long-term impacts through an entire generation is largely unknown. Here, we present the first whole generation study exposing fish to a 500 nm polystyrene plastic particle at environmentally relevant concentrations. Short- and long-term adverse effects were investigated in the zebrafish model organism using a holistic multi-omics approach. The particles accumulated in the yolk sac of young larvae and short-term biological impacts included immune-relevant gene regulation related to inflammation and tolerance as well as disruption of metabolic processes, such as the fatty acid and lipid pathways. The long-term effects comprised gene regulations pointing towards skin and/or gill inflammation, dysbiosis of the gut microbiota, a tendency towards decreased condition factor in adult males as well as a lowered reproductive capability. From this study, it can be concluded that exposures to plastic nanoparticles have an impact on population as well as ecosystem level in fish and likely also in other vertebrates.

KW - Immune effects

KW - Metabolic disruption

KW - Microbiota dysbiosis

KW - Nutritional condition

KW - Polystyrene

U2 - 10.1016/j.jhazmat.2021.127705

DO - 10.1016/j.jhazmat.2021.127705

M3 - Journal article

C2 - 34802818

AN - SCOPUS:85119403823

VL - 424

JO - Journal of Hazardous Materials

JF - Journal of Hazardous Materials

SN - 0304-3894

IS - Part D

M1 - 127705

ER -