Proteomic evidence of dietary sources in ancient dental calculus
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Proteomic evidence of dietary sources in ancient dental calculus. / Hendy, Jessica; Warinner, Christina; Bouwman, Abigail; Collins, Matthew James; Fiddyment, Sarah; Fischer, Roman; Hagan, Richard; Hofman, Courtney A; Holst, Malin; Chaves, Eros; Klaus, Lauren; Larson, Greger; Mackie, Meaghan; McGrath, Krista; Mundorff, Amy Z; Radini, Anita; Rao, Huiyun; Trachsel, Christian; Velsko, Irina M; Speller, Camilla F.
In: Proceedings of the Royal Society B: Biological Sciences, Vol. 285, No. 1883, 977, 2018.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Proteomic evidence of dietary sources in ancient dental calculus
AU - Hendy, Jessica
AU - Warinner, Christina
AU - Bouwman, Abigail
AU - Collins, Matthew James
AU - Fiddyment, Sarah
AU - Fischer, Roman
AU - Hagan, Richard
AU - Hofman, Courtney A
AU - Holst, Malin
AU - Chaves, Eros
AU - Klaus, Lauren
AU - Larson, Greger
AU - Mackie, Meaghan
AU - McGrath, Krista
AU - Mundorff, Amy Z
AU - Radini, Anita
AU - Rao, Huiyun
AU - Trachsel, Christian
AU - Velsko, Irina M
AU - Speller, Camilla F
N1 - © 2018 The Authors.
PY - 2018
Y1 - 2018
N2 - Archaeological dental calculus has emerged as a rich source of ancient biomolecules, including proteins. Previous analyses of proteins extracted from ancient dental calculus revealed the presence of the dietary milk protein β-lactoglobulin, providing direct evidence of dairy consumption in the archaeological record. However, the potential for calculus to preserve other food-related proteins has not yet been systematically explored. Here we analyse shotgun metaproteomic data from 100 archaeological dental calculus samples ranging from the Iron Age to the post-medieval period (eighth century BC to nineteenth century AD) in England, as well as 14 dental calculus samples from contemporary dental patients and recently deceased individuals, to characterize the range and extent of dietary proteins preserved in dental calculus. In addition to milk proteins, we detect proteomic evidence of foodstuffs such as cereals and plant products, as well as the digestive enzyme salivary amylase. We discuss the importance of optimized protein extraction methods, data analysis approaches and authentication strategies in the identification of dietary proteins from archaeological dental calculus. This study demonstrates that proteomic approaches can robustly identify foodstuffs in the archaeological record that are typically under-represented due to their poor macroscopic preservation.
AB - Archaeological dental calculus has emerged as a rich source of ancient biomolecules, including proteins. Previous analyses of proteins extracted from ancient dental calculus revealed the presence of the dietary milk protein β-lactoglobulin, providing direct evidence of dairy consumption in the archaeological record. However, the potential for calculus to preserve other food-related proteins has not yet been systematically explored. Here we analyse shotgun metaproteomic data from 100 archaeological dental calculus samples ranging from the Iron Age to the post-medieval period (eighth century BC to nineteenth century AD) in England, as well as 14 dental calculus samples from contemporary dental patients and recently deceased individuals, to characterize the range and extent of dietary proteins preserved in dental calculus. In addition to milk proteins, we detect proteomic evidence of foodstuffs such as cereals and plant products, as well as the digestive enzyme salivary amylase. We discuss the importance of optimized protein extraction methods, data analysis approaches and authentication strategies in the identification of dietary proteins from archaeological dental calculus. This study demonstrates that proteomic approaches can robustly identify foodstuffs in the archaeological record that are typically under-represented due to their poor macroscopic preservation.
U2 - 10.1098/rspb.2018.0977
DO - 10.1098/rspb.2018.0977
M3 - Journal article
C2 - 30051838
VL - 285
JO - Proceedings of the Royal Society B: Biological Sciences
JF - Proceedings of the Royal Society B: Biological Sciences
SN - 0962-8452
IS - 1883
M1 - 977
ER -
ID: 200287936