Resolving a clinical tuberculosis outbreak using palaeogenomic genome reconstruction methodologies

Research output: Contribution to journalJournal articleResearchpeer-review

  • Rhys Jones
  • Sandoval-Velasco, Marcela
  • Llinos G. Harris
  • Sue Morgan
  • Mark Temple
  • Michael C. Ruddy
  • Rhian Williams
  • Michael D. Perry
  • Matt Hitchings
  • Thomas S. Wilkinson
  • Thomas Humphrey
  • Gilbert, Tom
  • Angharad P. Davies

This study describes the analysis of DNA from heat-killed (boilate) isolates of Mycobacterium tuberculosis from two UK outbreaks where DNA was of sub-optimal quality for the standard methodologies routinely used in microbial genomics. An Illumina library construction method developed for sequencing ancient DNA was successfully used to obtain whole genome sequences, allowing analysis of the outbreak by gene-by-gene MLST, SNP mapping and phylogenetic analysis. All cases were spoligotyped to the same Haarlem H1 sub-lineage. This is the first described application of ancient DNA library construction protocols to allow whole genome sequencing of a clinical tuberculosis outbreak. Using this method it is possible to obtain epidemiologically meaningful data even when DNA is of insufficient quality for standard methods.

Original languageEnglish
Article number101865
Number of pages5
Publication statusPublished - Dec 2019

    Research areas

  • Ancient DNA library construction, Mycobacterium tuberculosis, Outbreak investigation, Palaeogenomics, Whole genome sequencing

ID: 230200977