Shotgun environmental DNA, pollen, and macrofossil analysis of lateglacial lake sediments from southern Sweden
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Shotgun environmental DNA, pollen, and macrofossil analysis of lateglacial lake sediments from southern Sweden. / Parducci, Laura; Alsos, Inger Greve; Unneberg, Per; Pedersen, Mikkel W.; Han, Lu; Lammers, Youri; Salonen, J. Sakari; Väliranta, Minna M.; Slotte, Tanja; Wohlfarth, Barbara.
In: Frontiers in Ecology and Evolution, Vol. 7, No. JUN, 189, 2019.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Shotgun environmental DNA, pollen, and macrofossil analysis of lateglacial lake sediments from southern Sweden
AU - Parducci, Laura
AU - Alsos, Inger Greve
AU - Unneberg, Per
AU - Pedersen, Mikkel W.
AU - Han, Lu
AU - Lammers, Youri
AU - Salonen, J. Sakari
AU - Väliranta, Minna M.
AU - Slotte, Tanja
AU - Wohlfarth, Barbara
PY - 2019
Y1 - 2019
N2 - The lake sediments of Hässeldala Port in south-east Sweden provide an archive of local and regional environmental conditions ~14.5-9.5 ka BP (thousand years before present) and allow testing DNA sequencing techniques to reconstruct past vegetation changes. We combined shotgun sequencing with plant micro- and macrofossil analyses to investigate sediments dating to the Allerød (14.1-12.7 ka BP), Younger Dryas (12.7-11.7 ka BP), and Preboreal (<11.7 ka BP). Number of reads and taxa were not associated with sample age or organic content. This suggests that, beyond the initial rapid degradation, DNA is still present. The proportion of recovered plant DNA was low, but allowed identifying an important number of plant taxa, thus adding valid information on the composition of the local vegetation. Importantly, DNA provides a stronger signal of plant community changes than plant micro- and plant macrofossil analyses alone, since a larger number of new taxa were recorded in Younger Dryas samples. A comparison between the three proxies highlights differences and similarities and supports earlier findings that plants growing close to or within a lake are recorded by DNA. Plant macrofossil remains moreover show that tree birch was present close to the ancient lake since the Allerød; together with the DNA results, this indicates that boreal to subarctic climatic conditions also prevailed during the cold Younger Dryas interval. Increasing DNA reference libraries and enrichment strategies prior to sequencing are necessary to improve the potential and accuracy of plant identification using the shotgun metagenomic approach.
AB - The lake sediments of Hässeldala Port in south-east Sweden provide an archive of local and regional environmental conditions ~14.5-9.5 ka BP (thousand years before present) and allow testing DNA sequencing techniques to reconstruct past vegetation changes. We combined shotgun sequencing with plant micro- and macrofossil analyses to investigate sediments dating to the Allerød (14.1-12.7 ka BP), Younger Dryas (12.7-11.7 ka BP), and Preboreal (<11.7 ka BP). Number of reads and taxa were not associated with sample age or organic content. This suggests that, beyond the initial rapid degradation, DNA is still present. The proportion of recovered plant DNA was low, but allowed identifying an important number of plant taxa, thus adding valid information on the composition of the local vegetation. Importantly, DNA provides a stronger signal of plant community changes than plant micro- and plant macrofossil analyses alone, since a larger number of new taxa were recorded in Younger Dryas samples. A comparison between the three proxies highlights differences and similarities and supports earlier findings that plants growing close to or within a lake are recorded by DNA. Plant macrofossil remains moreover show that tree birch was present close to the ancient lake since the Allerød; together with the DNA results, this indicates that boreal to subarctic climatic conditions also prevailed during the cold Younger Dryas interval. Increasing DNA reference libraries and enrichment strategies prior to sequencing are necessary to improve the potential and accuracy of plant identification using the shotgun metagenomic approach.
KW - Ancient DNA
KW - Environmental DNA
KW - Lake sediments
KW - Macrofossils remains
KW - Pollen
KW - Shotgun sequencing (metagenomics)
U2 - 10.3389/fevo.2019.00189
DO - 10.3389/fevo.2019.00189
M3 - Journal article
AN - SCOPUS:85068599790
VL - 7
JO - Frontiers in Ecology and Evolution
JF - Frontiers in Ecology and Evolution
SN - 2296-701X
IS - JUN
M1 - 189
ER -
ID: 254994655