Tensile properties of human collagen fibrils and fascicles are insensitive to environmental salts
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Tensile properties of human collagen fibrils and fascicles are insensitive to environmental salts. / Svensson, René B; Hassenkam, Tue; Grant, Colin A; Magnusson, S Peter.
In: Biophysical Journal, Vol. 99, No. 12, 15.12.2010, p. 4020-7.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Tensile properties of human collagen fibrils and fascicles are insensitive to environmental salts
AU - Svensson, René B
AU - Hassenkam, Tue
AU - Grant, Colin A
AU - Magnusson, S Peter
N1 - Copyright © 2010 Biophysical Society. Published by Elsevier Inc. All rights reserved.
PY - 2010/12/15
Y1 - 2010/12/15
N2 - To carry out realistic in vitro mechanical testing on anatomical tissue, a choice has to be made regarding the buffering environment. Therefore, it is important to understand how the environment may influence the measurement to ensure the highest level of accuracy. The most physiologically relevant loading direction of tendon is along its longitudinal axis. Thus, in this study, we focus on the tensile mechanical properties of two hierarchical levels from human patellar tendon, namely: individual collagen fibrils and fascicles. Investigations on collagen fibrils and fascicles were made at pH 7.4 in solutions of phosphate-buffered saline at three different concentrations as well as two HEPES buffered solutions containing NaCl or NaCl + CaCl2. An atomic force microscope technique was used for tensile testing of individual collagen fibrils. Only a slight increase in relative energy dissipation was observed at the highest phosphate-buffered saline concentration for both the fibrils and fascicles, indicating a stabilizing effect of ionic screening, but changes were much less than reported for radial compression. Due to the small magnitude of the effects, the tensile mechanical properties of collagen fibrils and fascicles from the patellar tendon of mature humans are essentially insensitive to environmental salt concentration and composition at physiological pH.
AB - To carry out realistic in vitro mechanical testing on anatomical tissue, a choice has to be made regarding the buffering environment. Therefore, it is important to understand how the environment may influence the measurement to ensure the highest level of accuracy. The most physiologically relevant loading direction of tendon is along its longitudinal axis. Thus, in this study, we focus on the tensile mechanical properties of two hierarchical levels from human patellar tendon, namely: individual collagen fibrils and fascicles. Investigations on collagen fibrils and fascicles were made at pH 7.4 in solutions of phosphate-buffered saline at three different concentrations as well as two HEPES buffered solutions containing NaCl or NaCl + CaCl2. An atomic force microscope technique was used for tensile testing of individual collagen fibrils. Only a slight increase in relative energy dissipation was observed at the highest phosphate-buffered saline concentration for both the fibrils and fascicles, indicating a stabilizing effect of ionic screening, but changes were much less than reported for radial compression. Due to the small magnitude of the effects, the tensile mechanical properties of collagen fibrils and fascicles from the patellar tendon of mature humans are essentially insensitive to environmental salt concentration and composition at physiological pH.
KW - Calcium Chloride
KW - Fibrillar Collagens
KW - Humans
KW - Microscopy, Atomic Force
KW - Patellar Ligament
KW - Sodium Chloride
KW - Solutions
KW - Stress, Mechanical
KW - Tensile Strength
U2 - 10.1016/j.bpj.2010.11.018
DO - 10.1016/j.bpj.2010.11.018
M3 - Journal article
C2 - 21156145
VL - 99
SP - 4020
EP - 4027
JO - Biophysical Journal
JF - Biophysical Journal
SN - 0006-3495
IS - 12
ER -
ID: 33248213