The transcriptional and regulatory identity of erythropoietin producing cells

Research output: Contribution to journal › Journal article › Research › peer-review

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The transcriptional and regulatory identity of erythropoietin producing cells. / Kragesteen, Bjørt K.; Giladi, Amir; David, Eyal; Halevi, Shahar; Geirsdóttir, Laufey; Lempke, Olga M.; Li, Baoguo; Bapst, Andreas M.; Xie, Ken; Katzenelenbogen, Yonatan; Dahl, Sophie L.; Sheban, Fadi; Gurevich-Shapiro, Anna; Zada, Mor; Phan, Truong San; Avellino, Roberto; Wang, Shuang-Yin; Barboy, Oren; Shlomi-Loubaton, Shir; Winning, Sandra; Markwerth, Philipp P.; Dekalo, Snir; Keren-Shaul, Hadas; Kedmi, Merav; Sikora, Martin; Fandrey, Joachim; Korneliussen, Thorfinn S.; Prchal, Josef T.; Rosenzweig, Barak; Yutkin, Vladimir; Racimo, Fernando; Willerslev, Eske; Gur, Chamutal; Wenger, Roland H.; Amit, Ido.

In: Nature Medicine, Vol. 29, No. 5, 2023, p. 1191-1200.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kragesteen, BK, Giladi, A, David, E, Halevi, S, Geirsdóttir, L, Lempke, OM, Li, B, Bapst, AM, Xie, K, Katzenelenbogen, Y, Dahl, SL, Sheban, F, Gurevich-Shapiro, A, Zada, M, Phan, TS, Avellino, R, Wang, S-Y, Barboy, O, Shlomi-Loubaton, S, Winning, S, Markwerth, PP, Dekalo, S, Keren-Shaul, H, Kedmi, M, Sikora, M, Fandrey, J, Korneliussen, TS, Prchal, JT, Rosenzweig, B, Yutkin, V, Racimo, F, Willerslev, E, Gur, C, Wenger, RH & Amit, I 2023, 'The transcriptional and regulatory identity of erythropoietin producing cells', Nature Medicine, vol. 29, no. 5, pp. 1191-1200. https://doi.org/10.1038/s41591-023-02314-7

APA

Kragesteen, B. K., Giladi, A., David, E., Halevi, S., Geirsdóttir, L., Lempke, O. M., Li, B., Bapst, A. M., Xie, K., Katzenelenbogen, Y., Dahl, S. L., Sheban, F., Gurevich-Shapiro, A., Zada, M., Phan, T. S., Avellino, R., Wang, S-Y., Barboy, O., Shlomi-Loubaton, S., ... Amit, I. (2023). The transcriptional and regulatory identity of erythropoietin producing cells. Nature Medicine, 29(5), 1191-1200. https://doi.org/10.1038/s41591-023-02314-7

Vancouver

Kragesteen BK, Giladi A, David E, Halevi S, Geirsdóttir L, Lempke OM et al. The transcriptional and regulatory identity of erythropoietin producing cells. Nature Medicine. 2023;29(5):1191-1200. https://doi.org/10.1038/s41591-023-02314-7

Author

Kragesteen, Bjørt K. ; Giladi, Amir ; David, Eyal ; Halevi, Shahar ; Geirsdóttir, Laufey ; Lempke, Olga M. ; Li, Baoguo ; Bapst, Andreas M. ; Xie, Ken ; Katzenelenbogen, Yonatan ; Dahl, Sophie L. ; Sheban, Fadi ; Gurevich-Shapiro, Anna ; Zada, Mor ; Phan, Truong San ; Avellino, Roberto ; Wang, Shuang-Yin ; Barboy, Oren ; Shlomi-Loubaton, Shir ; Winning, Sandra ; Markwerth, Philipp P. ; Dekalo, Snir ; Keren-Shaul, Hadas ; Kedmi, Merav ; Sikora, Martin ; Fandrey, Joachim ; Korneliussen, Thorfinn S. ; Prchal, Josef T. ; Rosenzweig, Barak ; Yutkin, Vladimir ; Racimo, Fernando ; Willerslev, Eske ; Gur, Chamutal ; Wenger, Roland H. ; Amit, Ido. / The transcriptional and regulatory identity of erythropoietin producing cells. In: Nature Medicine. 2023 ; Vol. 29, No. 5. pp. 1191-1200.

Bibtex

@article{a1ecefd714de4d47a031f90e46b6b52b,

title = "The transcriptional and regulatory identity of erythropoietin producing cells",

abstract = "Erythropoietin (Epo) is the master regulator of erythropoiesis and oxygen homeostasis. Despite its physiological importance, the molecular and genomic contexts of the cells responsible for renal Epo production remain unclear, limiting more-effective therapies for anemia. Here, we performed single-cell RNA and transposase-accessible chromatin (ATAC) sequencing of an Epo reporter mouse to molecularly identify Epo-producing cells under hypoxic conditions. Our data indicate that a distinct population of kidney stroma, which we term Norn cells, is the major source of endocrine Epo production in mice. We use these datasets to identify the markers, signaling pathways and transcriptional circuits characteristic of Norn cells. Using single-cell RNA sequencing and RNA in situ hybridization in human kidney tissues, we further provide evidence that this cell population is conserved in humans. These preliminary findings open new avenues to functionally dissect EPO gene regulation in health and disease and may serve as groundwork to improve erythropoiesis-stimulating therapies.",

author = "Kragesteen, {Bj{\o}rt K.} and Amir Giladi and Eyal David and Shahar Halevi and Laufey Geirsd{\'o}ttir and Lempke, {Olga M.} and Baoguo Li and Bapst, {Andreas M.} and Ken Xie and Yonatan Katzenelenbogen and Dahl, {Sophie L.} and Fadi Sheban and Anna Gurevich-Shapiro and Mor Zada and Phan, {Truong San} and Roberto Avellino and Shuang-Yin Wang and Oren Barboy and Shir Shlomi-Loubaton and Sandra Winning and Markwerth, {Philipp P.} and Snir Dekalo and Hadas Keren-Shaul and Merav Kedmi and Martin Sikora and Joachim Fandrey and Korneliussen, {Thorfinn S.} and Prchal, {Josef T.} and Barak Rosenzweig and Vladimir Yutkin and Fernando Racimo and Eske Willerslev and Chamutal Gur and Wenger, {Roland H.} and Ido Amit",

note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive licence to Springer Nature America, Inc.",

year = "2023",

doi = "10.1038/s41591-023-02314-7",

language = "English",

volume = "29",

pages = "1191--1200",

journal = "Nature Medicine",

issn = "1078-8956",

publisher = "nature publishing group",

number = "5",

}

RIS

TY - JOUR

T1 - The transcriptional and regulatory identity of erythropoietin producing cells

AU - Kragesteen, Bjørt K.

AU - Giladi, Amir

AU - David, Eyal

AU - Halevi, Shahar

AU - Geirsdóttir, Laufey

AU - Lempke, Olga M.

AU - Li, Baoguo

AU - Bapst, Andreas M.

AU - Xie, Ken

AU - Katzenelenbogen, Yonatan

AU - Dahl, Sophie L.

AU - Sheban, Fadi

AU - Gurevich-Shapiro, Anna

AU - Zada, Mor

AU - Phan, Truong San

AU - Avellino, Roberto

AU - Wang, Shuang-Yin

AU - Barboy, Oren

AU - Shlomi-Loubaton, Shir

AU - Winning, Sandra

AU - Markwerth, Philipp P.

AU - Dekalo, Snir

AU - Keren-Shaul, Hadas

AU - Kedmi, Merav

AU - Sikora, Martin

AU - Fandrey, Joachim

AU - Korneliussen, Thorfinn S.

AU - Prchal, Josef T.

AU - Rosenzweig, Barak

AU - Yutkin, Vladimir

AU - Racimo, Fernando

AU - Willerslev, Eske

AU - Gur, Chamutal

AU - Wenger, Roland H.

AU - Amit, Ido

PY - 2023

Y1 - 2023

N2 - Erythropoietin (Epo) is the master regulator of erythropoiesis and oxygen homeostasis. Despite its physiological importance, the molecular and genomic contexts of the cells responsible for renal Epo production remain unclear, limiting more-effective therapies for anemia. Here, we performed single-cell RNA and transposase-accessible chromatin (ATAC) sequencing of an Epo reporter mouse to molecularly identify Epo-producing cells under hypoxic conditions. Our data indicate that a distinct population of kidney stroma, which we term Norn cells, is the major source of endocrine Epo production in mice. We use these datasets to identify the markers, signaling pathways and transcriptional circuits characteristic of Norn cells. Using single-cell RNA sequencing and RNA in situ hybridization in human kidney tissues, we further provide evidence that this cell population is conserved in humans. These preliminary findings open new avenues to functionally dissect EPO gene regulation in health and disease and may serve as groundwork to improve erythropoiesis-stimulating therapies.

AB - Erythropoietin (Epo) is the master regulator of erythropoiesis and oxygen homeostasis. Despite its physiological importance, the molecular and genomic contexts of the cells responsible for renal Epo production remain unclear, limiting more-effective therapies for anemia. Here, we performed single-cell RNA and transposase-accessible chromatin (ATAC) sequencing of an Epo reporter mouse to molecularly identify Epo-producing cells under hypoxic conditions. Our data indicate that a distinct population of kidney stroma, which we term Norn cells, is the major source of endocrine Epo production in mice. We use these datasets to identify the markers, signaling pathways and transcriptional circuits characteristic of Norn cells. Using single-cell RNA sequencing and RNA in situ hybridization in human kidney tissues, we further provide evidence that this cell population is conserved in humans. These preliminary findings open new avenues to functionally dissect EPO gene regulation in health and disease and may serve as groundwork to improve erythropoiesis-stimulating therapies.

U2 - 10.1038/s41591-023-02314-7

DO - 10.1038/s41591-023-02314-7

M3 - Journal article

C2 - 37106166

AN - SCOPUS:85153740022

VL - 29

SP - 1191

EP - 1200

JO - Nature Medicine

JF - Nature Medicine

SN - 1078-8956

IS - 5

ER -

ID: 346453069

Globe Institute