Vertebrate environmental DNA from leaf swabs

Research output: Contribution to journal › Comment/debate › Research › peer-review

Standard

Vertebrate environmental DNA from leaf swabs. / Lynggaard, Christina; Calvignac-Spencer, Sébastien; Chapman, Colin A.; Kalbitzer, Urs; Leendertz, Fabian H.; Omeja, Patrick A.; Opito, Emmanuel A.; Sarkar, Dipto; Bohmann, Kristine; Gogarten, Jan F.

In: Current Biology, Vol. 33, No. 16, 2023, p. R853-R854.

Research output: Contribution to journal › Comment/debate › Research › peer-review

Harvard

Lynggaard, C, Calvignac-Spencer, S, Chapman, CA, Kalbitzer, U, Leendertz, FH, Omeja, PA, Opito, EA, Sarkar, D, Bohmann, K & Gogarten, JF 2023, 'Vertebrate environmental DNA from leaf swabs', Current Biology, vol. 33, no. 16, pp. R853-R854. https://doi.org/10.1016/j.cub.2023.06.031

APA

Lynggaard, C., Calvignac-Spencer, S., Chapman, C. A., Kalbitzer, U., Leendertz, F. H., Omeja, P. A., Opito, E. A., Sarkar, D., Bohmann, K., & Gogarten, J. F. (2023). Vertebrate environmental DNA from leaf swabs. Current Biology, 33(16), R853-R854. https://doi.org/10.1016/j.cub.2023.06.031

Vancouver

Lynggaard C, Calvignac-Spencer S, Chapman CA, Kalbitzer U, Leendertz FH, Omeja PA et al. Vertebrate environmental DNA from leaf swabs. Current Biology. 2023;33(16):R853-R854. https://doi.org/10.1016/j.cub.2023.06.031

Author

Lynggaard, Christina ; Calvignac-Spencer, Sébastien ; Chapman, Colin A. ; Kalbitzer, Urs ; Leendertz, Fabian H. ; Omeja, Patrick A. ; Opito, Emmanuel A. ; Sarkar, Dipto ; Bohmann, Kristine ; Gogarten, Jan F. / Vertebrate environmental DNA from leaf swabs. In: Current Biology. 2023 ; Vol. 33, No. 16. pp. R853-R854.

Bibtex

@article{b5c60f0cc3354ef5a43145e83aa6ede1,
title = "Vertebrate environmental DNA from leaf swabs",
abstract = "Terrestrial vertebrates are threatened by anthropogenic activities around the world. The rapid biodiversity loss that ensues is most intense in the tropics and affects ecosystem functions, such as seed dispersal, or may facilitate pathogen transmission1. Monitoring vertebrate distributions is essential for understanding changes in biodiversity and ecosystems and also for adaptive management strategies. Environmental DNA (eDNA) approaches have the potential to play a key role in such efforts. Here, we explore whether eDNA swabbed from terrestrial vegetation in a tropical biodiversity hotspot is a useful tool for vertebrate biomonitoring. By swabbing leaves, we collected eDNA from 24 swabs at three locations in Kibale National Park, Uganda and used two metabarcoding systems to catalog the vertebrate taxa in the samples. We detected 52 wild vertebrate genera, including 26 avian and 24 mammalian genera; 30 of these assignments could be refined to the species level. We detected an average of 7.6 genera per swab. This approach, with its inexpensive and simple collection and DNA extraction, opens the door for inexpensive large-scale vertebrate biomonitoring.",
author = "Christina Lynggaard and S{\'e}bastien Calvignac-Spencer and Chapman, {Colin A.} and Urs Kalbitzer and Leendertz, {Fabian H.} and Omeja, {Patrick A.} and Opito, {Emmanuel A.} and Dipto Sarkar and Kristine Bohmann and Gogarten, {Jan F.}",
note = "Publisher Copyright: {\textcopyright} 2023 The Authors",
year = "2023",
doi = "10.1016/j.cub.2023.06.031",
language = "English",
volume = "33",
pages = "R853--R854",
journal = "Current Biology",
issn = "0960-9822",
publisher = "Cell Press",
number = "16",

}

RIS

TY - JOUR

T1 - Vertebrate environmental DNA from leaf swabs

AU - Lynggaard, Christina

AU - Calvignac-Spencer, Sébastien

AU - Chapman, Colin A.

AU - Kalbitzer, Urs

AU - Leendertz, Fabian H.

AU - Omeja, Patrick A.

AU - Opito, Emmanuel A.

AU - Sarkar, Dipto

AU - Bohmann, Kristine

AU - Gogarten, Jan F.

N1 - Publisher Copyright: © 2023 The Authors

PY - 2023

Y1 - 2023

N2 - Terrestrial vertebrates are threatened by anthropogenic activities around the world. The rapid biodiversity loss that ensues is most intense in the tropics and affects ecosystem functions, such as seed dispersal, or may facilitate pathogen transmission1. Monitoring vertebrate distributions is essential for understanding changes in biodiversity and ecosystems and also for adaptive management strategies. Environmental DNA (eDNA) approaches have the potential to play a key role in such efforts. Here, we explore whether eDNA swabbed from terrestrial vegetation in a tropical biodiversity hotspot is a useful tool for vertebrate biomonitoring. By swabbing leaves, we collected eDNA from 24 swabs at three locations in Kibale National Park, Uganda and used two metabarcoding systems to catalog the vertebrate taxa in the samples. We detected 52 wild vertebrate genera, including 26 avian and 24 mammalian genera; 30 of these assignments could be refined to the species level. We detected an average of 7.6 genera per swab. This approach, with its inexpensive and simple collection and DNA extraction, opens the door for inexpensive large-scale vertebrate biomonitoring.

AB - Terrestrial vertebrates are threatened by anthropogenic activities around the world. The rapid biodiversity loss that ensues is most intense in the tropics and affects ecosystem functions, such as seed dispersal, or may facilitate pathogen transmission1. Monitoring vertebrate distributions is essential for understanding changes in biodiversity and ecosystems and also for adaptive management strategies. Environmental DNA (eDNA) approaches have the potential to play a key role in such efforts. Here, we explore whether eDNA swabbed from terrestrial vegetation in a tropical biodiversity hotspot is a useful tool for vertebrate biomonitoring. By swabbing leaves, we collected eDNA from 24 swabs at three locations in Kibale National Park, Uganda and used two metabarcoding systems to catalog the vertebrate taxa in the samples. We detected 52 wild vertebrate genera, including 26 avian and 24 mammalian genera; 30 of these assignments could be refined to the species level. We detected an average of 7.6 genera per swab. This approach, with its inexpensive and simple collection and DNA extraction, opens the door for inexpensive large-scale vertebrate biomonitoring.

U2 - 10.1016/j.cub.2023.06.031

DO - 10.1016/j.cub.2023.06.031

M3 - Comment/debate

C2 - 37607478

AN - SCOPUS:85168316642

VL - 33

SP - R853-R854

JO - Current Biology

JF - Current Biology

SN - 0960-9822

IS - 16

ER -

ID: 363440696