The isolation of nucleic acids from fixed, paraffin-embedded tissues-which methods are useful when?

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The isolation of nucleic acids from fixed, paraffin-embedded tissues-which methods are useful when? / Gilbert, M Thomas P; Haselkorn, Tamara; Bunce, Michael; Sanchez, Juan J; Lucas, Sebastian B; Jewell, Laurence D; Van Marck, Eric; Worobey, Michael.

In: PLoS ONE, Vol. 2, No. 6, 2007, p. e537.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Gilbert, MTP, Haselkorn, T, Bunce, M, Sanchez, JJ, Lucas, SB, Jewell, LD, Van Marck, E & Worobey, M 2007, 'The isolation of nucleic acids from fixed, paraffin-embedded tissues-which methods are useful when?', PLoS ONE, vol. 2, no. 6, pp. e537. https://doi.org/10.1371/journal.pone.0000537

APA

Gilbert, M. T. P., Haselkorn, T., Bunce, M., Sanchez, J. J., Lucas, S. B., Jewell, L. D., Van Marck, E., & Worobey, M. (2007). The isolation of nucleic acids from fixed, paraffin-embedded tissues-which methods are useful when? PLoS ONE, 2(6), e537. https://doi.org/10.1371/journal.pone.0000537

Vancouver

Gilbert MTP, Haselkorn T, Bunce M, Sanchez JJ, Lucas SB, Jewell LD et al. The isolation of nucleic acids from fixed, paraffin-embedded tissues-which methods are useful when? PLoS ONE. 2007;2(6):e537. https://doi.org/10.1371/journal.pone.0000537

Author

Gilbert, M Thomas P ; Haselkorn, Tamara ; Bunce, Michael ; Sanchez, Juan J ; Lucas, Sebastian B ; Jewell, Laurence D ; Van Marck, Eric ; Worobey, Michael. / The isolation of nucleic acids from fixed, paraffin-embedded tissues-which methods are useful when?. In: PLoS ONE. 2007 ; Vol. 2, No. 6. pp. e537.

Bibtex

@article{c2908770149411ddbee902004c4f4f50,
title = "The isolation of nucleic acids from fixed, paraffin-embedded tissues-which methods are useful when?",
abstract = "Museums and pathology collections around the world represent an archive of genetic material to study populations and diseases. For preservation purposes, a large portion of these collections has been fixed in formalin-containing solutions, a treatment that results in cross-linking of biomolecules. Cross-linking not only complicates isolation of nucleic acid but also introduces polymerase {"}blocks{"} during PCR. A wide variety of methods exists for the recovery of DNA and RNA from archival tissues, and although a number of previous studies have qualitatively compared the relative merits of the different techniques, very few have undertaken wide scale quantitative comparisons. To help address this issue, we have undertaken a study that investigates the quality of nucleic acids recovered from a test panel of fixed specimens that have been manipulated following a number of the published protocols. These include methods of pre-treating the samples prior to extraction, extraction and nucleic acid purification methods themselves, and a post-extraction enzymatic repair technique. We find that although many of the published methods have distinct positive effects on some characteristics of the nucleic acids, the benefits often come at a cost. In addition, a number of the previously published techniques appear to have no effect at all. Our findings recommend that the extraction methodology adopted should be chosen carefully. Here we provide a quick reference table that can be used to determine appropriate protocols for particular aims.",
author = "Gilbert, {M Thomas P} and Tamara Haselkorn and Michael Bunce and Sanchez, {Juan J} and Lucas, {Sebastian B} and Jewell, {Laurence D} and {Van Marck}, Eric and Michael Worobey",
year = "2007",
doi = "10.1371/journal.pone.0000537",
language = "English",
volume = "2",
pages = "e537",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "6",

}

RIS

TY - JOUR

T1 - The isolation of nucleic acids from fixed, paraffin-embedded tissues-which methods are useful when?

AU - Gilbert, M Thomas P

AU - Haselkorn, Tamara

AU - Bunce, Michael

AU - Sanchez, Juan J

AU - Lucas, Sebastian B

AU - Jewell, Laurence D

AU - Van Marck, Eric

AU - Worobey, Michael

PY - 2007

Y1 - 2007

N2 - Museums and pathology collections around the world represent an archive of genetic material to study populations and diseases. For preservation purposes, a large portion of these collections has been fixed in formalin-containing solutions, a treatment that results in cross-linking of biomolecules. Cross-linking not only complicates isolation of nucleic acid but also introduces polymerase "blocks" during PCR. A wide variety of methods exists for the recovery of DNA and RNA from archival tissues, and although a number of previous studies have qualitatively compared the relative merits of the different techniques, very few have undertaken wide scale quantitative comparisons. To help address this issue, we have undertaken a study that investigates the quality of nucleic acids recovered from a test panel of fixed specimens that have been manipulated following a number of the published protocols. These include methods of pre-treating the samples prior to extraction, extraction and nucleic acid purification methods themselves, and a post-extraction enzymatic repair technique. We find that although many of the published methods have distinct positive effects on some characteristics of the nucleic acids, the benefits often come at a cost. In addition, a number of the previously published techniques appear to have no effect at all. Our findings recommend that the extraction methodology adopted should be chosen carefully. Here we provide a quick reference table that can be used to determine appropriate protocols for particular aims.

AB - Museums and pathology collections around the world represent an archive of genetic material to study populations and diseases. For preservation purposes, a large portion of these collections has been fixed in formalin-containing solutions, a treatment that results in cross-linking of biomolecules. Cross-linking not only complicates isolation of nucleic acid but also introduces polymerase "blocks" during PCR. A wide variety of methods exists for the recovery of DNA and RNA from archival tissues, and although a number of previous studies have qualitatively compared the relative merits of the different techniques, very few have undertaken wide scale quantitative comparisons. To help address this issue, we have undertaken a study that investigates the quality of nucleic acids recovered from a test panel of fixed specimens that have been manipulated following a number of the published protocols. These include methods of pre-treating the samples prior to extraction, extraction and nucleic acid purification methods themselves, and a post-extraction enzymatic repair technique. We find that although many of the published methods have distinct positive effects on some characteristics of the nucleic acids, the benefits often come at a cost. In addition, a number of the previously published techniques appear to have no effect at all. Our findings recommend that the extraction methodology adopted should be chosen carefully. Here we provide a quick reference table that can be used to determine appropriate protocols for particular aims.

U2 - 10.1371/journal.pone.0000537

DO - 10.1371/journal.pone.0000537

M3 - Journal article

C2 - 17579711

VL - 2

SP - e537

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 6

ER -

ID: 3848462