A glimpse of the paleome in endolithic microbial communities

Research output: Working paper › Preprint › Research

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A glimpse of the paleome in endolithic microbial communities. / Wegner, Carl-Eric; Stahl, Raphaela; Velsko, Irina; Hübner, Alex; Fagernäs, Zandra; Warinner, Christina; Lehmann, Robert; Ritschel, Thomas; Totsche, Kai U.; Küsel, Kirsten.

bioRxiv, 2022.

Research output: Working paper › Preprint › Research

Harvard

Wegner, C-E, Stahl, R, Velsko, I, Hübner, A, Fagernäs, Z, Warinner, C, Lehmann, R, Ritschel, T, Totsche, KU & Küsel, K 2022 'A glimpse of the paleome in endolithic microbial communities' bioRxiv. https://doi.org/10.1101/2022.09.23.509128

APA

Wegner, C-E., Stahl, R., Velsko, I., Hübner, A., Fagernäs, Z., Warinner, C., Lehmann, R., Ritschel, T., Totsche, K. U., & Küsel, K. (2022). A glimpse of the paleome in endolithic microbial communities. bioRxiv. https://doi.org/10.1101/2022.09.23.509128

Vancouver

Wegner C-E, Stahl R, Velsko I, Hübner A, Fagernäs Z, Warinner C et al. A glimpse of the paleome in endolithic microbial communities. bioRxiv. 2022. https://doi.org/10.1101/2022.09.23.509128

Author

Wegner, Carl-Eric ; Stahl, Raphaela ; Velsko, Irina ; Hübner, Alex ; Fagernäs, Zandra ; Warinner, Christina ; Lehmann, Robert ; Ritschel, Thomas ; Totsche, Kai U. ; Küsel, Kirsten. / A glimpse of the paleome in endolithic microbial communities. bioRxiv, 2022.

Bibtex

@techreport{1ca6172811934eba9a6aee67cd9b6b0b,
title = "A glimpse of the paleome in endolithic microbial communities",
abstract = "The terrestrial subsurface houses a significant proportion of the Earth{\textquoteright}s microbial biomass. Our understanding about terrestrial subsurface microbiomes is almost exclusively derived from groundwater and porous sediments. To obtain more insights about endolithic microbiomes and their metabolic status, we investigated rock samples from the vadose zone, fractured aquifers, and deep aquitards. Using methods from paleogenomics, we recovered sufficient DNA for metagenomics from rock specimens independent of porosity, lithology, and depth. We estimated between 2.81 and 4.25 × 105 cells × g−1 rock. DNA damage patterns revealed paleome signatures (genetic records of past microbial communities) for three rock specimens from the vadose zone. The taxonomy and functional potential of paleome communities revealed increased abundances of chemolithoautotrophs, and a broader metabolic potential for aromatic hydrocarbon breakdown. Our study suggests that limestones represent ideal archives for genetic records of past microbial communities, due to their specific conditions facilitating long-term DNA preservation.",
author = "Carl-Eric Wegner and Raphaela Stahl and Irina Velsko and Alex H{\"u}bner and Zandra Fagern{\"a}s and Christina Warinner and Robert Lehmann and Thomas Ritschel and Totsche, {Kai U.} and Kirsten K{\"u}sel",
year = "2022",
doi = "10.1101/2022.09.23.509128",
language = "English",
publisher = "bioRxiv",
type = "WorkingPaper",
institution = "bioRxiv",

}

RIS

TY - UNPB

T1 - A glimpse of the paleome in endolithic microbial communities

AU - Wegner, Carl-Eric

AU - Stahl, Raphaela

AU - Velsko, Irina

AU - Hübner, Alex

AU - Fagernäs, Zandra

AU - Warinner, Christina

AU - Lehmann, Robert

AU - Ritschel, Thomas

AU - Totsche, Kai U.

AU - Küsel, Kirsten

PY - 2022

Y1 - 2022

N2 - The terrestrial subsurface houses a significant proportion of the Earth’s microbial biomass. Our understanding about terrestrial subsurface microbiomes is almost exclusively derived from groundwater and porous sediments. To obtain more insights about endolithic microbiomes and their metabolic status, we investigated rock samples from the vadose zone, fractured aquifers, and deep aquitards. Using methods from paleogenomics, we recovered sufficient DNA for metagenomics from rock specimens independent of porosity, lithology, and depth. We estimated between 2.81 and 4.25 × 105 cells × g−1 rock. DNA damage patterns revealed paleome signatures (genetic records of past microbial communities) for three rock specimens from the vadose zone. The taxonomy and functional potential of paleome communities revealed increased abundances of chemolithoautotrophs, and a broader metabolic potential for aromatic hydrocarbon breakdown. Our study suggests that limestones represent ideal archives for genetic records of past microbial communities, due to their specific conditions facilitating long-term DNA preservation.

AB - The terrestrial subsurface houses a significant proportion of the Earth’s microbial biomass. Our understanding about terrestrial subsurface microbiomes is almost exclusively derived from groundwater and porous sediments. To obtain more insights about endolithic microbiomes and their metabolic status, we investigated rock samples from the vadose zone, fractured aquifers, and deep aquitards. Using methods from paleogenomics, we recovered sufficient DNA for metagenomics from rock specimens independent of porosity, lithology, and depth. We estimated between 2.81 and 4.25 × 105 cells × g−1 rock. DNA damage patterns revealed paleome signatures (genetic records of past microbial communities) for three rock specimens from the vadose zone. The taxonomy and functional potential of paleome communities revealed increased abundances of chemolithoautotrophs, and a broader metabolic potential for aromatic hydrocarbon breakdown. Our study suggests that limestones represent ideal archives for genetic records of past microbial communities, due to their specific conditions facilitating long-term DNA preservation.

U2 - 10.1101/2022.09.23.509128

DO - 10.1101/2022.09.23.509128

M3 - Preprint

BT - A glimpse of the paleome in endolithic microbial communities

PB - bioRxiv

ER -

ID: 339134004