Next-generation sequencing of multiple individuals per barcoded library by deconvolution of sequenced amplicons using endonuclease fragment analysis

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Next-generation sequencing of multiple individuals per barcoded library by deconvolution of sequenced amplicons using endonuclease fragment analysis. / Andersen, Jeppe D; Pereira, Vania; Pietroni, Carlotta; Mikkelsen, Martin; Johansen, Peter; Børsting, Claus; Morling, Niels.

In: BioTechniques, Vol. 57, No. 2, 08.2014, p. 91-94.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Andersen, JD, Pereira, V, Pietroni, C, Mikkelsen, M, Johansen, P, Børsting, C & Morling, N 2014, 'Next-generation sequencing of multiple individuals per barcoded library by deconvolution of sequenced amplicons using endonuclease fragment analysis', BioTechniques, vol. 57, no. 2, pp. 91-94. https://doi.org/10.2144/000114200

APA

Andersen, J. D., Pereira, V., Pietroni, C., Mikkelsen, M., Johansen, P., Børsting, C., & Morling, N. (2014). Next-generation sequencing of multiple individuals per barcoded library by deconvolution of sequenced amplicons using endonuclease fragment analysis. BioTechniques, 57(2), 91-94. https://doi.org/10.2144/000114200

Vancouver

Andersen JD, Pereira V, Pietroni C, Mikkelsen M, Johansen P, Børsting C et al. Next-generation sequencing of multiple individuals per barcoded library by deconvolution of sequenced amplicons using endonuclease fragment analysis. BioTechniques. 2014 Aug;57(2):91-94. https://doi.org/10.2144/000114200

Author

Andersen, Jeppe D ; Pereira, Vania ; Pietroni, Carlotta ; Mikkelsen, Martin ; Johansen, Peter ; Børsting, Claus ; Morling, Niels. / Next-generation sequencing of multiple individuals per barcoded library by deconvolution of sequenced amplicons using endonuclease fragment analysis. In: BioTechniques. 2014 ; Vol. 57, No. 2. pp. 91-94.

Bibtex

@article{a4b7897f29e54da898dc06964ca58d06,
title = "Next-generation sequencing of multiple individuals per barcoded library by deconvolution of sequenced amplicons using endonuclease fragment analysis",
abstract = "The simultaneous sequencing of samples from multiple individuals increases the efficiency of next-generation sequencing (NGS) while also reducing costs. Here we describe a novel and simple approach for sequencing DNA from multiple individuals per barcode. Our strategy relies on the endonuclease digestion of PCR amplicons prior to library preparation, creating a specific fragment pattern for each individual that can be resolved after sequencing. By using both barcodes and restriction fragment patterns, we demonstrate the ability to sequence the human melanocortin 1 receptor (MC1R) genes from 72 individuals using only 24 barcoded libraries.",
author = "Andersen, {Jeppe D} and Vania Pereira and Carlotta Pietroni and Martin Mikkelsen and Peter Johansen and Claus B{\o}rsting and Niels Morling",
year = "2014",
month = aug,
doi = "10.2144/000114200",
language = "English",
volume = "57",
pages = "91--94",
journal = "BioTechniques",
issn = "0736-6205",
publisher = "Informa Healthcare",
number = "2",

}

RIS

TY - JOUR

T1 - Next-generation sequencing of multiple individuals per barcoded library by deconvolution of sequenced amplicons using endonuclease fragment analysis

AU - Andersen, Jeppe D

AU - Pereira, Vania

AU - Pietroni, Carlotta

AU - Mikkelsen, Martin

AU - Johansen, Peter

AU - Børsting, Claus

AU - Morling, Niels

PY - 2014/8

Y1 - 2014/8

N2 - The simultaneous sequencing of samples from multiple individuals increases the efficiency of next-generation sequencing (NGS) while also reducing costs. Here we describe a novel and simple approach for sequencing DNA from multiple individuals per barcode. Our strategy relies on the endonuclease digestion of PCR amplicons prior to library preparation, creating a specific fragment pattern for each individual that can be resolved after sequencing. By using both barcodes and restriction fragment patterns, we demonstrate the ability to sequence the human melanocortin 1 receptor (MC1R) genes from 72 individuals using only 24 barcoded libraries.

AB - The simultaneous sequencing of samples from multiple individuals increases the efficiency of next-generation sequencing (NGS) while also reducing costs. Here we describe a novel and simple approach for sequencing DNA from multiple individuals per barcode. Our strategy relies on the endonuclease digestion of PCR amplicons prior to library preparation, creating a specific fragment pattern for each individual that can be resolved after sequencing. By using both barcodes and restriction fragment patterns, we demonstrate the ability to sequence the human melanocortin 1 receptor (MC1R) genes from 72 individuals using only 24 barcoded libraries.

U2 - 10.2144/000114200

DO - 10.2144/000114200

M3 - Journal article

C2 - 25109295

VL - 57

SP - 91

EP - 94

JO - BioTechniques

JF - BioTechniques

SN - 0736-6205

IS - 2

ER -

ID: 122825752